TBU Publications
Repository of TBU Publications

Evaluation of genetic diversity of edible honeysuckle monitored by rapd in relation to bioactive substances

DSpace Repository

Show simple item record


dc.title Evaluation of genetic diversity of edible honeysuckle monitored by rapd in relation to bioactive substances en
dc.contributor.author Cehula, Marcela
dc.contributor.author Juríková, Tünde
dc.contributor.author Žiarovská, Jana
dc.contributor.author Mlček, Jiří
dc.contributor.author Kysel', Matúš
dc.relation.ispartof Potravinarstvo Slovak Journal of Food Sciences
dc.identifier.issn 1338-0230 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2019
utb.relation.volume 13
utb.relation.issue 1
dc.citation.spage 490
dc.citation.epage 496
dc.type article
dc.language.iso en
dc.publisher HACCP Consulting
dc.identifier.doi 10.5219/1139
dc.relation.uri http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1139/836
dc.subject DPPH en
dc.subject Folin-Ciocalteu en
dc.subject Honeysuckle en
dc.subject RAPD en
dc.subject Total polyphenols content en
dc.description.abstract The aim of this study was clarifying the relation between genetic diversity of edible honeysuckle (Lonicera kamtschatica) and the major group of biologically active substances as total polyphenols content (TPC) including antioxidant activity (AO). Fruits of edible honeysuckle becomes more and more popular, especially in Europe. The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed.The biological material including 14 cultivars of the edible honeysuckle ('Zoluška', 'Amfora', 'Pruhonický 44', 'Vasilijevsky', 'Moskovskaja', 'Vojtek', 'Sinoglaska', 'Altaj', 'Lipnická', 'Kamčadalka', 'Sinaja Ptica', 'Fialka', 'Modrý Triumf', and 'Leningradský velikán') originated from Czech republic (Žabcice near Brno). The content of TPC and AO were determined by location and its soil-climatic conditions and these environmental circumstances determines the RAPD profiles of analysed honeysuckle acessions, too. DPPH method was used to analyze AO and Folin-Ciocalteu method was used to determine TPC. The results of experiment showed that the highest value of AO was determined at the cultivars 'Zoluška' (81.04 mg.L-1) and the lowest was measured in 'Kamčadalka' (54.122 mg.L-1). On the contrary, the highest content of TPC was determined at the cultivar 'Kamčadalka' (51.09 mg.L-1) and the lowest value was measured at the cultivar 'Pruhonický 44' (21.65 mg.L-1). Phylogenetic trees were similar in genetic distance. The content of TPC and AO were not statistically significant in relation to cultivar. The analyzed cultivars of the edible honeysuckle were separated in 4 clusters according to used primers. In both gel images, the amplicon size ranged from 100 to 1,500 bp. We found that genetic diversity was partially related to content of total polyphenolic substances and antioxidant activity. Based on phylogenetic trees we have stated that variety 'Lipnická', 'Sinoglaska', 'Altaj', 'Leningradský velikán', 'Modrý Triumf', 'Sinaja Ptica' and 'Kamčadalka' were grouped in the similar cluster. The highest genetic distance was determined at the variety 'Vasilijevskaja' and 'Amfora'. In the same way, there were variety 'Vojtek', 'Fialka' and 'Zoluška'. © 2019 Potravinarstvo Slovak Journal of Food Sciences. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1008805
utb.identifier.obdid 43880524
utb.identifier.scopus 2-s2.0-85067284656
utb.source j-scopus
dc.date.accessioned 2019-08-13T10:17:22Z
dc.date.available 2019-08-13T10:17:22Z
dc.rights Attribution 3.0 International
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.rights.access openAccess
utb.ou Department of Food Analysis and Chemistry
utb.contributor.internalauthor Mlček, Jiří
utb.fulltext.affiliation Marcela Cehula, Tünde Juríková, Jana Žiarovská, Jiří Mlček, Matúš Kyseľ * Marcela Cehula, Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Botany and Genetics, Nábrežie mládeže 91, 949 74 Nitra, Slovak Republic, Tel.: +421376408581, E-mail: phd178564@ukf.sk ORCID: https://orcid.org/0000-0002-8048-1144 Tünde Juríková, Constantine the Philosopher University, Faculty of Central European Studies, Institute for Teacher Training, Dražovská 4, 949 74 Nitra, Slovakia, Tel.: +421376408 855, E-mail: tjurikova@ukf.sk ORCID: https://orcid.org/0000-0002-8286-8262 Jana Žiarovská, Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414244, E-mail: jana.ziarovska@uniag.sk ORCID: https://orcid.org/0000-0002-0005-9729 Jiří Mlček, Tomas Bata University in Zlín, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic, Tel.: +420576033030, E-mail: mlcek@utb.cz ORCID: https://orcid.org/0000-0002-5753-8560 Matúš Kyseľ, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421907045799, E-mail: mat.kysel@gmail.com ORCID: https://orcid.org/0000-0002-1679-391X Corresponding author: *
utb.fulltext.dates Received: 28 May 2019 Accepted: 30 May 2019 Available online: 28 June 2019
utb.fulltext.sponsorship This work was supported by KEGA 012UKF-4/2019 and was supported by European Community under project no 26220220180: Building Research Centre "AgroBioTech".
utb.scopus.affiliation Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Botany and Genetics, Nábrežie mládeže 91, Nitra, 949 74, Slovakia; Constantine the Philosopher University, Faculty of Central European Studies, Institute for Teacher Training, Dražovská 4, Nitra, 949 74, Slovakia; Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, Nitra, 949 76, Slovakia; Tomas Bata University in Zlín, Faculty of Technology, Department of Food Analysis and Chemistry, Nám. T.G. Masaryka 5555, Zlín, 760 01, Czech Republic; Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, Nitra, 949 76, Slovakia
utb.fulltext.projects KEGA 012UKF-4/2019
utb.fulltext.projects 26220220180
utb.fulltext.faculty Faculty of Technology
utb.fulltext.ou Department of Food Analysis and Chemistry
Find Full text

Files in this item

Show simple item record

Attribution 3.0 International Except where otherwise noted, this item's license is described as Attribution 3.0 International