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Assessment of genetic diversity of edible honeysuckle monitored through RAPD in relative to bioactive substances

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dc.title Assessment of genetic diversity of edible honeysuckle monitored through RAPD in relative to bioactive substances en
dc.contributor.author Cehula, Marcela
dc.contributor.author Mlček, Jiří
dc.contributor.author Juríková, Tünde
dc.contributor.author Žiarovská, Jana
dc.contributor.author Paulen, Oleg
dc.contributor.author Dokoupil, Libor
dc.contributor.author Adámková, Anna
dc.contributor.author Babosova, Ramona
dc.relation.ispartof Agronomy-Basel
dc.date.issued 2020
utb.relation.volume 10
utb.relation.issue 6
dc.type article
dc.language.iso en
dc.publisher Mdpi
dc.identifier.doi 10.3390/agronomy10060868
dc.relation.uri https://www.mdpi.com/2073-4395/10/6/868
dc.subject honeysuckle en
dc.subject DNA polymorphism en
dc.subject TAA en
dc.subject TPC en
dc.description.abstract This study aimed to analyze the genetic diversity of selected cultivars and clones ofLonicerasp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits ofLonicerasp. in 2018-2019 was performed. Applied statistical methods evaluating TPC and TAA were: correlation and regression, ANOVA, and agglomerative hierarchical clustering. The neighbor-joining method and gel images analysis (PyElph software, Pavel and Vasile, Bucharest, Romania) were used for genetic analysis. The average TPC of all cultivars and clones was 5.32 mg L(-1)in the year 2018 and 3.78 mg L(-1)in 2019. The average TAA values of all samples in 2018 were 8.24 mg L-1, and 9.27 mg L(-1)in 2019. Statistically significant correlations between years and cultivars and clones were found. Based on the lengths and number of fragments by random amplified polymorphic DNA (RAPD)-PCR's, it was found that 'clone 5' and 'clone 7' were genetically close to the grown cultivars from the Czech Republic. Therefore, edible honeysuckle fruits are a valuable source of important nutrients for the human body and its health. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1009855
utb.identifier.scopus 2-s2.0-85090667996
utb.identifier.wok 000553988500001
utb.source J-wok
dc.date.accessioned 2020-08-21T08:57:15Z
dc.date.available 2020-08-21T08:57:15Z
dc.description.sponsorship TBU in Zlin [IGA/FT/2020/010]; AgroBioTech Research Centre [ITMS 26220220180]; MZe C. R [206553/2011-17253]
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Department of Food Analysis and Chemistry
utb.contributor.internalauthor Mlček, Jiří
utb.fulltext.sponsorship This article was supported by the internal grant of TBU in Zlín [No. IGA/FT/2020/010] and by AgroBioTech Research Centre, built in accordance with the project Building “AgroBioTech” Research Centre ITMS 26220220180 and by MZeČR 206553/2011-17253” Národní program konzervace a využívání genetických zdroju° rostlin a agrobiodiversity”.
utb.wos.affiliation [Cehula, Marcela] Constantine Philosopher Univ Nitra, Fac Nat Sci, Dept Bot & Genet, Nabrezie Mladeze 91, Nitra 94974, Slovakia; [Mlcek, Jiri; Adamkova, Anna] Tomas Bata Univ Zlin, Fac Technol, Dept Food Anal & Chem, Vavreckova 275, Zlin 76001, Czech Republic; [Jurikova, Tunde] Constantine Philosopher Univ Nitra, Fac Cent European Studies, Inst Teacher Training, Drazovska 4, Nitra 94974, Slovakia; [Ziarovska, Jana] Slovak Univ Agr, Fac Agrobiol & Food Resources, Dept Genet & Plant Breeding, Trieda A Hlinku 2, Nitra 94976, Slovakia; [Paulen, Oleg] Slovak Univ Agr, Hort & Landscape Engn Fac, Trieda A Hlinku 2, Nitra 94976, Slovakia; [Dokoupil, Libor] Mendel Univ Brno, Fac Hort, Dept Breeding & Propagat Hort Plants, Valticka 337, Lednice 69144, Czech Republic; [Babosova, Ramona] Constantine Philosopher Univ Nitra, Fac Nat Sci, Dept Zool & Anthropol, Nabrezie Mladeze 91, Nitra 94974, Slovakia
utb.scopus.affiliation Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, Nitra, 949 74, Slovakia; Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, Zlín, 760 01, Czech Republic; Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Drazovská 4, Nitra, 949 74, Slovakia; Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Trieda A. Hlinku 2, Nitra, 949 76, Slovakia; Horticulture and Landscape Engineering Faculty, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, Nitra, 949 76, Slovakia; Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Valtická 337, Lednice, 691 44, Czech Republic; Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, Nitra, 949 74, Slovakia
utb.fulltext.projects IGA/FT/2020/010
utb.fulltext.projects ITMS 26220220180
utb.fulltext.projects 206553/2011-17253
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