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Comparison of Various Easy-to-Use Procedures for Extraction of Phenols from Apricot Fruits

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dc.title Comparison of Various Easy-to-Use Procedures for Extraction of Phenols from Apricot Fruits en
dc.contributor.author Zítka, Ondřej
dc.contributor.author Sochor, Jiří
dc.contributor.author Rop, Otakar
dc.contributor.author Skaličková, Sylvie
dc.contributor.author Šobrová, Pavlína
dc.contributor.author Zehnálek, Josef
dc.contributor.author Beklová, Miroslava
dc.contributor.author Krška, Boris
dc.contributor.author Adam, Vojtěch
dc.contributor.author Kizek, René
dc.relation.ispartof Molecules
dc.identifier.issn 1420-3049 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2011-04
utb.relation.volume 16
utb.relation.issue 4
dc.citation.spage 2914
dc.citation.epage 2936
dc.type article
dc.language.iso en
dc.publisher MDPI AG en
dc.identifier.doi 10.3390/molecules16042914
dc.relation.uri http://www.mdpi.com/1420-3049/16/4/2914/
dc.subject polyphenols en
dc.subject apricot en
dc.subject high performance liquid chromatography en
dc.subject CoulArray electrochemical detector en
dc.subject UV-VIS detector en
dc.description.abstract Phenols are broadly distributed in the plant kingdom and are the most abundant secondary metabolites of plants. Plant polyphenols have drawn increasing attention due to their potential antioxidant properties and their marked effects in the prevention of various oxidative stress associated diseases such as cancer. The objective of this study was to investigate a suitable method for determination of protocatechuic acid, 4-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferulic acid, quercetin, resveratrol and quercitrin from apricot samples. A high-performance liquid chromatograph with electrochemical and UV detectors was used. The method was optimized in respect to both the separation selectivity of individual phenolic compounds and the maximum sensitivity with the electrochemical detection. The lowest limits of detection (3 S/N) using UV detection were estimated for ferulic acid (3 mu M), quercitrin (4 mu M) and quercetin (4 mu M). Using electrochemical detection values of 27 nM, 40 nM and 37 nM were achieved for ferulic acid, quercitrin and quercetin, respectively. It follows from the acquired results that the coulometric detection under a universal potential of 600 mV is more suitable and sensitive for polyphenols determination than UV detection at a universal wavelength of 260 nm. Subsequently, we tested the influence of solvent composition, vortexing and sonication on separation efficiency. Our results showed that a combination of water, acetone and methanol in 20: 20: 60 ratio was the most effective for p-aminobenzoic acid, chlorgenic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, resveratrol and quercetin, in comparison with other solvents. On the other hand, vortexing at 4 C produced the highest yield. Moreover, we tested the contents of individual polyphenols in the apricot cultivars Mamaria, Mold and LE-1075. The major phenolic compounds were chlorgenic acid and rutin. Chlorgenic acid was found in amounts of 2,302 mg/100 g in cultivar LE-1075, 546 mg/100 g in cultivar Mamaria and 129 mg/100 g in cultivar Mold. Generally, the cultivar LE-1075 produced the highest polyphenol content values, contrary to Mold, which compared to cultivar LE-1075 was quite poor from the point of view of the phenolics content. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1002176
utb.identifier.rivid RIV/70883521:28110/11:43865340!RIV12-MSM-28110___
utb.identifier.obdid 43865342
utb.identifier.scopus 2-s2.0-79955532496
utb.identifier.wok 000289236200013
utb.identifier.coden MOLEF
utb.source j-wok
dc.date.accessioned 2011-08-16T15:06:36Z
dc.date.available 2011-08-16T15:06:36Z
dc.rights Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.access openAccess
utb.contributor.internalauthor Rop, Otakar
utb.fulltext.affiliation Ondrej Zitka 1, Jiri Sochor 1, Otakar Rop 2, Sylvie Skalickova 1, Pavlina Sobrova 1, Josef Zehnalek 1, Miroslava Beklova 3, Boris Krska 4, Vojtech Adam 1 and Rene Kizek 1,* 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlin, Namesti T. G. Masaryka 275, CZ-762 72 Zlin, Czech Republic 3 Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-61242 Brno, Czech Republic 4 Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic * Author to whom correspondence should be addressed; E-Mail: kizek@sci.muni.cz; Tel.: +420-5-4513-3350; Fax: +420-5-4521-2044.
utb.fulltext.dates Received: 9 March 2011; in revised form: 25 March 2011 / Accepted: 31 March 2011 / Published: 4 April 2011
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