Influence of extractive solvents on lipid and fatty acids content of edible freshwater algal and seaweed products, the green microalga Chlorella kessleri and the cyanobacterium Spirulina platensis

Ambrožová Vávra, Jarmila; Mišurcová, Ladislava; Vícha, Robert; Machů, Ludmila; Samek, Dušan; Baroň, Mojmír; Mlček, Jiří; Sochor, Jiří; Juríková, Tünde

dc.title	Influence of extractive solvents on lipid and fatty acids content of edible freshwater algal and seaweed products, the green microalga Chlorella kessleri and the cyanobacterium Spirulina platensis	en
dc.contributor.author	Ambrožová Vávra, Jarmila
dc.contributor.author	Mišurcová, Ladislava
dc.contributor.author	Vícha, Robert
dc.contributor.author	Machů, Ludmila
dc.contributor.author	Samek, Dušan
dc.contributor.author	Baroň, Mojmír
dc.contributor.author	Mlček, Jiří
dc.contributor.author	Sochor, Jiří
dc.contributor.author	Juríková, Tünde
dc.relation.ispartof	Molecules
dc.identifier.issn	1420-3049 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2014
utb.relation.volume	19
utb.relation.issue	2
dc.citation.spage	2344
dc.citation.epage	2360
dc.type	article
dc.language.iso	en
dc.publisher	MDPI AG	en
dc.identifier.doi	10.3390/molecules19022344
dc.relation.uri	http://www.mdpi.com/1420-3049/19/2/2344
dc.subject	Algae	en
dc.subject	Fatty acid	en
dc.subject	Lipid	en
dc.subject	Seaweed	en
dc.description.abstract	Total lipid contents of green (Chlorella pyrenoidosa, C), red (Porphyra tenera, N; Palmaria palmata, D), and brown (Laminaria japonica, K; Eisenia bicyclis, A; Undaria pinnatifida, W, WI; Hizikia fusiformis, H) commercial edible algal and cyanobacterial (Spirulina platensis, S) products, and autotrophically cultivated samples of the green microalga Chlorella kessleri (CK) and the cyanobacterium Spirulina platensis (SP) were determined using a solvent mixture of methanol/chloroform/water (1:2:1, v/v/v, solvent I) and n-hexane (solvent II). Total lipid contents ranged from 0.64% (II) to 18.02% (I) by dry weight and the highest total lipid content was observed in the autotrophically cultivated cyanobacterium Spirulina platensis. Solvent mixture I was found to be more effective than solvent II. Fatty acids were determined by gas chromatography of their methyl esters (% of total FAMEs). Generally, the predominant fatty acids (all results for extractions with solvent mixture I) were saturated palmitic acid (C16:0; 24.64%-65.49%), monounsaturated oleic acid (C18:1(n-9); 2.79%-26.45%), polyunsaturated linoleic acid (C18:2(n-6); 0.71%-36.38%), α-linolenic acid (C18:3(n-3); 0.00%-21.29%), γ-linolenic acid (C18:3(n-6); 1.94%-17.36%), and arachidonic acid (C20:4(n-6); 0.00%-15.37%). The highest content of ω-3 fatty acids (21.29%) was determined in Chlorella pyrenoidosa using solvent I, while conversely, the highest content of ω-6 fatty acids (41.42%) was observed in Chlorella kessleri using the same solvent. © 2014 by the authors; licensee MDPI, Basel, Switzerland.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1003695
utb.identifier.obdid	43871762
utb.identifier.scopus	2-s2.0-84894628589
utb.identifier.wok	000334418200061
utb.identifier.coden	MOLEF
utb.source	j-scopus
dc.date.accessioned	2014-03-13T16:50:51Z
dc.date.available	2014-03-13T16:50:51Z
dc.description.sponsorship	Postdocs in Biological Sciences at MENDELU [CZ.1.07/2.3.00/30.0017]; TBU in Zlin [IGA/FT/2014/011]
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.access	openAccess
utb.contributor.internalauthor	Ambrožová Vávra, Jarmila
utb.contributor.internalauthor	Mišurcová, Ladislava
utb.contributor.internalauthor	Vícha, Robert
utb.contributor.internalauthor	Machů, Ludmila
utb.contributor.internalauthor	Samek, Dušan
utb.contributor.internalauthor	Mlček, Jiří
utb.fulltext.affiliation	Jarmila Vavra Ambrozova 1, Ladislava Misurcova 1,, Robert Vicha 2, Ludmila Machu 1, Dusan Samek 1, Mojmir Baron 3, Jiri Mlcek 1, Jiri Sochor 3 and Tunde Jurikova 4 1 Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlin, nam. T. G. Masaryka 5555, Zlin CZ-760 01, Czech Republic; E-Mails: ambrozova@ft.utb.cz (J.V.A.); lmachu@ft.utb.cz (L.M.); dsamek@ft.utb.cz (D.S.); mlcek@ft.utb.cz (J.M.) 2 Department of Chemistry, Faculty of Technology, Tomas Bata University in Zlin, nam. T. G. Masaryka 5555, Zlin CZ-760 01, Czech Republic; E-Mail: rvicha@ft.utb.cz 3 Department of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Valticka 337, Lednice CZ-691 44, Czech Republic; E-Mails: MojmirBaron@seznam.cz (M.B.); sochor.jirik@seznam.cz (J.S.) 4 Department of Natural and Informatics Sciences, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Drazovska 4, Nitra SK-949 74, Slovak Republic; E-Mail: tjurikova@ukf.sk Author to whom correspondence should be addressed; E-Mail: misurcova@ft.utb.cz; Tel.: +420-576-031-592, Fax: +420-577-210-172.
utb.fulltext.dates	Received: 6 December 2013; in revised form: 5 February 2014 / Accepted: 7 February 2014 / Published: 21 February 2014
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utb.fulltext.sponsorship	Jiri Sochor would like to express his thanks for CZ.1.07/2.3.00/30.0017 Postdocs in Biological Sciences at MENDELU support. This research was also supported by the internal grant of TBU in Zlin No. IGA/FT/2014/011. We gratefully acknowledge Jiri Masojidek and Magda Sergejevova from the Laboratory of Algal Biotechnology, Algatech Center, Institute of Microbiology, Academy of Science (Trebon, Czech Republic) for providing the samples of freshwater microalga and cyanobacterium.
utb.fulltext.projects	CZ.1.07/2.3.00/30.0017
utb.fulltext.projects	IGA/FT/2014/011