Self-assembled mumio-stabilized bioactive gel systems for topical therapeutics of rheumatoid arthritis: structural, rheological, cytocompatibility, and antimicrobial properties

Abstract

This study presents the development of salicylate polyacrylic copolymer gel systems incorporating mumio particulates as a bioactive agent for the topical treatment of rheumatoid arthritis. Using an experimental design, formulations were optimized based on mumio, salicylic acid, and polyacrylate copolymer ratios. Rheological behavior was assessed through frequency, temperature, and time sweep tests to evaluate shear response, stability, and application suitability. Spectral and morphological analyses confirmed the uniformity and surface characteristics of the gels. Antimicrobial activity was tested against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. Cytotoxicity was evaluated using NIH-3T3 mouse fibroblast cells. Results showed over 60% microbial inhibition after 24 hours and maintained cell viability above 70% at both 24 and 48 hours, indicating good biocompatibility. The gels also exhibited smooth texture, consistent bioactive dispersion, and non-irritating properties. Overall, these findings support the potential of mumio-loaded salicylate-polyacrylic gels as stable, biocompatible, and effective topical therapeutics for rheumatoid arthritis.