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Plasma mediated chlorhexidine immobilization onto polylactic acid surface via carbodiimide chemistry: Antibacterial and cytocompatibility assessment

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dc.title Plasma mediated chlorhexidine immobilization onto polylactic acid surface via carbodiimide chemistry: Antibacterial and cytocompatibility assessment en
dc.contributor.author Özaltin, Kadir
dc.contributor.author Di Martino, Antonio
dc.contributor.author Capáková, Zdenka
dc.contributor.author Lehocký, Marián
dc.contributor.author Humpolíček, Petr
dc.contributor.author Sáha, Tomáš
dc.contributor.author Veselá, Daniela
dc.contributor.author Mozetic, Miran
dc.contributor.author Sáha, Petr
dc.relation.ispartof Polymers
dc.identifier.issn 2073-4360 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2021
utb.relation.volume 13
utb.relation.issue 8
dc.type article
dc.language.iso en
dc.publisher MDPI AG
dc.identifier.doi 10.3390/polym13081201
dc.relation.uri https://www.mdpi.com/2073-4360/13/8/1201
dc.subject chlorhexidine en
dc.subject polylactic acid en
dc.subject biomaterial associated infection en
dc.subject plasma treatment en
dc.subject cytocompatibility en
dc.description.abstract The development of antibacterial materials has great importance in avoiding bacterial contamination and the risk of infection for implantable biomaterials. An antibacterial thin film coating on the surface via chemical bonding is a promising technique to keep native bulk material properties unchanged. However, most of the polymeric materials are chemically inert and highly hydrophobic, which makes chemical agent coating challenging Herein, immobilization of chlorhexidine, a broad-spectrum bactericidal cationic compound, onto the polylactic acid surface was performed in a multistep physicochemical method. Direct current plasma was used for surface functionalization, followed by carbodiimide chemistry to link the coupling reagents of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC) and N-Hydroxysuccinimide (NHs) to create a free bonding site to anchor the chlorhexidine. Surface characterizations were performed by water contact angle test, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). The antibacterial activity was tested using Staphylococcus aureus and Escherichia coli. Finally, in vitro cytocompatibility of the samples was studied using primary mouse embryonic fibroblast cells. It was found that all samples were cytocompatible and the best antibacterial performance observed was the Chlorhexidine immobilized sample after NHs activation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. en
utb.faculty University Institute
utb.faculty Faculty of Technology
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1010291
utb.identifier.obdid 43882523
utb.identifier.scopus 2-s2.0-85104185854
utb.identifier.wok 000644590500001
utb.source j-scopus
dc.date.accessioned 2021-04-30T19:22:35Z
dc.date.available 2021-04-30T19:22:35Z
dc.description.sponsorship Czech Science FoundationGrant Agency of the Czech Republic [19-16861S]; Ministry of Education, Youth and Sports of the Czech Republic-DKRVO [RP/CPS/2020/001]; DKRVO [RP/CPS/2020/005]; Slovenian Research AgencySlovenian Research Agency - Slovenia [L2-2616]
dc.description.sponsorship RP/CPS/2020/005; Ministerstvo Školství, Mládeže a Tělovýchovy, MŠMT: RP/CPS/2020/001; Grantová Agentura České Republiky, GA ČR: 19-16861S; Javna Agencija za Raziskovalno Dejavnost RS, ARRS: L2-2616
dc.rights Attribution 4.0 International
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Center of Polymer Systems
utb.ou Footwear Research Center
utb.contributor.internalauthor Özaltin, Kadir
utb.contributor.internalauthor Di Martino, Antonio
utb.contributor.internalauthor Capáková, Zdenka
utb.contributor.internalauthor Lehocký, Marián
utb.contributor.internalauthor Humpolíček, Petr
utb.contributor.internalauthor Sáha, Tomáš
utb.contributor.internalauthor Veselá, Daniela
utb.contributor.internalauthor Sáha, Petr
utb.fulltext.sponsorship This research was funded by the Czech Science Foundation (grant no. 19-16861S), Ministry of Education, Youth and Sports of the Czech Republic–DKRVO (RP/CPS/2020/001), DKRVO (RP/CPS/2020/005), and the Slovenian Research Agency (project L2-2616).
utb.wos.affiliation [Ozaltin, Kadir; Di Martino, Antonio; Capakova, Zdenka; Lehocky, Marian; Humpolicek, Petr; Vesela, Daniela; Saha, Petr] Tomas Bata Univ Zlin, Ctr Polymer Syst, Trida Tomase Bati 5678, Zlin 76001, Czech Republic; [Di Martino, Antonio] Tomsk Polytech Univ, Res Sch Chem & Appl Biomed Sci, Lenin Av 30, Tomsk 634050, Russia; [Lehocky, Marian; Humpolicek, Petr] Tomas Bata Univ Zlin, Fac Technol, Vavreckova 275, Zlin 76001, Czech Republic; [Saha, Tomas] Tomas Bata Univ Zlin, Univ Inst, Footwear Res Ctr, Ovcirnou 3685, Zlin 76001, Czech Republic; [Mozetic, Miran] Jozef Stefan Inst, Dept Surface Engn & Optoelect, Jamova Cesta 39, Ljubljana 1000, Slovenia
utb.scopus.affiliation Center of Polymer Systems, Tomas Bata University in Zlin, Trida Tomase Bati 5678, Zlin, 760 01, Czech Republic; Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Lenin Av. 30, Tomsk, 634050, Russian Federation; Faculty of Technology, Tomas Bata University in Zlin, Vavreckova 275, Zlin, 760 01, Czech Republic; Footwear Research Center, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou 3685, Zlin, 760 01, Czech Republic; Department of Surface Engineering and Optoelectronics, Jozef Stefan Institute, Jamova Cesta 39, Ljubljana, 1000, Slovenia
utb.fulltext.projects 19-16861S
utb.fulltext.projects RP/CPS/2020/001
utb.fulltext.projects RP/CPS/2020/005
utb.fulltext.projects L2-2616
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International