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Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei

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dc.title Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei en
dc.contributor.author Pištěková, Hana
dc.contributor.author Jančová, Petra
dc.contributor.author Berčíková, Lucie
dc.contributor.author Buňka, František
dc.contributor.author Sokolová, Iveta
dc.contributor.author Šopík, Tomáš
dc.contributor.author Maršálková, Kristýna
dc.contributor.author Amaral, Olga Maria Reis Pacheco De
dc.contributor.author Buňková, Leona
dc.relation.ispartof Food Microbiology
dc.identifier.issn 0740-0020 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2020
utb.relation.volume 91
dc.type article
dc.language.iso en
dc.publisher Elsevier
dc.identifier.doi 10.1016/j.fm.2020.103550
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0740002020301398
dc.subject Biogenic amines degradation en
dc.subject Histamine en
dc.subject Lactobacillus casei en
dc.subject Primers en
dc.subject qPCR en
dc.description.abstract Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we designed two sets of primers for the detection and quantification of the multicopper oxidase gene (MCO), which encodes an enzyme involved in BAs degradation, and endogenous (glyceraldehyde-3-phosphate dehydrogenase) gene (GAPDH) in Lactobacillus casei group by real-time PCR (qPCR). We tested 15 Lactobacillus strains in the screening assays (thus, MCO gene possessing assay (PCR) and monitoring of BAs degradation by HPLC-UV), in which Lactobacillus casei CCDM 198 exhibited the best degradation abilities. For this strain, we monitored the expression of the target gene (MCO) in time (qPCR), the effect of redox treatments (cysteine, ascorbic acid) on the expression of the gene, and the ability to degrade BAs not only in a modified MRS medium (MRS/2) but also in a real food sample (milk). Moreover, decarboxylase activity (ability to form BAs) of this strain was excluded. According to the results, CCDM 198 significantly (P < 0.05) reduced BAs (putrescine, histamine, tyramine, cadaverine), up to 25% decline in 48 h. The highest level of relative expression of MCO (5.21 ± 0.14) was achieved in MRS/2 media with cysteine. © 2020 Elsevier Ltd en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1009719
utb.identifier.obdid 43881671
utb.identifier.scopus 2-s2.0-85085551999
utb.identifier.wok 000540697600008
utb.identifier.pubmed 32539976
utb.identifier.coden FOMIE
utb.source j-scopus
dc.date.accessioned 2020-06-24T08:32:13Z
dc.date.available 2020-06-24T08:32:13Z
dc.description.sponsorship Grant Agency of the Czech Republic (GACR)Grant Agency of the Czech Republic [17.09594S]; Internal Grant of TBU in Zlin [IGA/FT/2020/009]
utb.contributor.internalauthor Pištěková, Hana
utb.contributor.internalauthor Jančová, Petra
utb.contributor.internalauthor Berčíková, Lucie
utb.contributor.internalauthor Buňka, František
utb.contributor.internalauthor Sokolová, Iveta
utb.contributor.internalauthor Šopík, Tomáš
utb.contributor.internalauthor Maršálková, Kristýna
utb.contributor.internalauthor Buňková, Leona
utb.fulltext.affiliation Hana Pištěková a, Petra Jančová a∗, Lucie Berčíková a, František Buňka b, Iveta Sokolová a, Tomáš Šopík b, Kristýna Maršálková a, Olga Maria Reis Pacheco de Amaral c, Leona Buňková a a Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic b Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic c Department of Technologies and Applied Sciences, Escola Superior Agária, Instituto Politécnico de Beja, Rua Pedro Soares S/N, Apartado 6155, 7800-295, Beja, Portugal ∗ Corresponding author. E-mail address: jancova@utb.cz (P. Jančová).
utb.fulltext.dates Received 18 October 2019 Received in revised form 12 February 2020 Accepted 20 May 2020 Available online 23 May 2020
utb.fulltext.sponsorship The financial support from the Grant Agency of the Czech Republic (GAČR No. 17˗09594S ) and Internal Grant of TBU in Zlín (No. IGA/FT/2020/009 ) is greatly acknowledged
utb.wos.affiliation [Pistekova, Hana; Jancova, Petra; Bercikova, Lucie; Sokolova, Iveta; Marsalkova, Kristyna; Bunkova, Leona] Tomas Bata Univ Zlin, Fac Technol, Dept Environm Protect Engn, Vavreckova 275, Zlin 76001, Czech Republic; [Bunka, Frantisek; Sopik, Tomas] Tomas Bata Univ Zlin, Fac Technol, Dept Food Technol, Vavreckova 275, Zlin 76001, Czech Republic; [Reis Pacheco de Amaral, Olga Maria] Inst Politecn Beja, Dept Technol & Appl Sci, Escola Super Agaria, Rua Pedro Soares S-N,Apartado 6155, P-7800295 Beja, Portugal[Pistekova, Hana; Jancova, Petra; Bercikova, Lucie; Sokolova, Iveta; Marsalkova, Kristyna; Bunkova, Leona] Tomas Bata Univ Zlin, Fac Technol, Dept Environm Protect Engn, Vavreckova 275, Zlin 76001, Czech Republic; [Bunka, Frantisek; Sopik, Tomas] Tomas Bata Univ Zlin, Fac Technol, Dept Food Technol, Vavreckova 275, Zlin 76001, Czech Republic; [Reis Pacheco de Amaral, Olga Maria] Inst Politecn Beja, Dept Technol & Appl Sci, Escola Super Agaria, Rua Pedro Soares S-N,Apartado 6155, P-7800295 Beja, Portugal
utb.scopus.affiliation Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, Zlín, 76001, Czech Republic; Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, Zlín, 76001, Czech Republic; Department of Technologies and Applied Sciences, Escola Superior Agária, Instituto Politécnico de Beja, Rua Pedro Soares S/N, Apartado 6155, Beja, 7800-295, Portugal
utb.fulltext.projects 17˗09594S
utb.fulltext.projects IGA/FT/2020/009
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty Faculty of Technology
utb.fulltext.ou Department of Environmental Protection Engineering
utb.fulltext.ou Department of Environmental Protection Engineering
utb.fulltext.ou Department of Environmental Protection Engineering
utb.fulltext.ou Department of Food Technology
utb.fulltext.ou Department of Environmental Protection Engineering
utb.fulltext.ou Department of Food Technology
utb.fulltext.ou Department of Environmental Protection Engineering
utb.fulltext.ou Department of Environmental Protection Engineering
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