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Tomato root growth inhibition by salinity and cadmium is mediated by S-nitrosative modifications of ROS metabolic enzymes controlled by S-nitrosoglutathione reductase

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dc.title Tomato root growth inhibition by salinity and cadmium is mediated by S-nitrosative modifications of ROS metabolic enzymes controlled by S-nitrosoglutathione reductase en
dc.contributor.author Jedelská, Tereza
dc.contributor.author Kraiczová, Veronika Šmotková
dc.contributor.author Berčíková, Lucie
dc.contributor.author Činčalová, Lucie
dc.contributor.author Luhová, Lenka
dc.contributor.author Petřivalský, Marek
dc.relation.ispartof Biomolecules
dc.identifier.issn 2218-273X Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2019
utb.relation.volume 9
utb.relation.issue 9
dc.type article
dc.language.iso en
dc.publisher MDPI
dc.identifier.doi 10.3390/biom9090393
dc.relation.uri https://www.mdpi.com/2218-273X/9/9/393
dc.subject abiotic stress en
dc.subject cadmium en
dc.subject nitric oxide en
dc.subject reactive oxygen species en
dc.subject root growth en
dc.subject S-nitrosation en
dc.subject S-nitrosoglutathione reductase en
dc.subject salinity en
dc.subject Solanum habrochaites en
dc.subject Solanum lycopersicum en
dc.description.abstract S-nitrosoglutathione reductase (GSNOR) exerts crucial roles in the homeostasis of nitric oxide (NO) and reactive nitrogen species (RNS) in plant cells through indirect control of S-nitrosation, an important protein post-translational modification in signaling pathways of NO. Using cultivated and wild tomato species, we studied GSNOR function in interactions of key enzymes of reactive oxygen species (ROS) metabolism with RNS mediated by protein S-nitrosation during tomato root growth and responses to salinity and cadmium. Application of a GSNOR inhibitor N6022 increased both NO and S-nitrosothiol levels and stimulated root growth in both genotypes. Moreover, N6022 treatment, as well as S-nitrosoglutathione (GSNO) application, caused intensive S-nitrosation of important enzymes of ROS metabolism, NADPH oxidase (NADPHox) and ascorbate peroxidase (APX). Under abiotic stress, activities of APX and NADPHox were modulated by S-nitrosation. Increased production of H2O2 and subsequent oxidative stress were observed in wild Solanumhabrochaites, together with increased GSNOR activity and reduced S-nitrosothiols. An opposite effect occurred in cultivated S. lycopersicum, where reduced GSNOR activity and intensive S-nitrosation resulted in reduced ROS levels by abiotic stress. These data suggest stress-triggered disruption of ROS homeostasis, mediated by modulation of RNS and S-nitrosation of NADPHox and APX, underlies tomato root growth inhibition by salinity and cadmium stress. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1009095
utb.identifier.obdid 43880580
utb.identifier.scopus 2-s2.0-85071443648
utb.identifier.wok 000489102800005
utb.identifier.pubmed 31438648
utb.source j-scopus
dc.date.accessioned 2019-09-19T07:56:16Z
dc.date.available 2019-09-19T07:56:16Z
dc.description.sponsorship Palacky University in Olomouc [IGA_2019_022]
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Department of Environmental Protection Engineering
utb.contributor.internalauthor Berčíková, Lucie
utb.fulltext.affiliation Tereza Jedelská 1, Veronika Šmotková Kraiczová 1,2, Lucie Berčíková 1,3, Lucie Činčálová 1, Lenka Luhová 1, Marek Petřivalský 1* 1 Department of Biochemistry, Faculty of Science, Palacký University, CZ-783 71 Olomouc, Czech Republic 2 Present address: Department of Immunology, Faculty of Medicine and Dentistry, Palacký University, CZ-77900 Olomouc, Czech Republic 3 Present address: Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, 760 01 Zlín, Czech Republic * Correspondence: marek.petrivalsky@upol.cz
utb.fulltext.dates Received: 28 June 2019 Accepted: 19 August 2019 Published: 21 August 2019
utb.fulltext.sponsorship This research was funded by Palacký University in Olomouc (IGA_2019_022).
utb.wos.affiliation [Jedelska, Tereza; Kraiczova, Veronika Smotkova; Bercikova, Lucie; Cincalova, Lucie; Luhova, Lenka; Petrivalsky, Marek] Palacky Univ, Fac Sci, Dept Biochem, CZ-78371 Olomouc, Czech Republic; [Kraiczova, Veronika Smotkova] Palacky Univ, Dept Immunol, Fac Med & Dent, CZ-77900 Olomouc, Czech Republic; [Bercikova, Lucie] Tomas Bata Univ Zlin, Fac Technol, Dept Environm Protect Engn, Zlin 76001, Czech Republic
utb.scopus.affiliation Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, CZ-783 71, Czech Republic; Present address: Department of Immunology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, CZ-77900, Czech Republic; Present address: Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, 760 01 Zlín, Czech Republic
utb.fulltext.projects IGA_2019_022
utb.fulltext.faculty Faculty of Technology
utb.fulltext.ou Department of Environmental Protection Engineering
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