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Antibacterial activity and cytotoxicity of immobilized glucosamine/chondroitin sulfate on polylactic acid films

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dc.title Antibacterial activity and cytotoxicity of immobilized glucosamine/chondroitin sulfate on polylactic acid films en
dc.contributor.author Karakurt, Ilkay
dc.contributor.author Özaltin, Kadir
dc.contributor.author Veselá, Daniela
dc.contributor.author Lehocký, Marián
dc.contributor.author Humpolíček, Petr
dc.contributor.author Mozetič, Miran
dc.relation.ispartof Polymers
dc.identifier.issn 2073-4360 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2019
utb.relation.volume 11
utb.relation.issue 7
dc.type article
dc.language.iso en
dc.publisher MDPI AG
dc.identifier.doi 10.3390/polym11071186
dc.relation.uri https://www.mdpi.com/2073-4360/11/7/1186
dc.subject plasma treatment en
dc.subject surface modification en
dc.subject saccharide immobilization en
dc.subject antibacterial activity en
dc.subject count-plate method en
dc.subject cytotoxicity en
dc.subject chondroitin sulfate en
dc.description.abstract Polylactic acid (PLA) is one of the most produced polymeric materials, due to its exceptional chemical and mechanical properties. Some of them, such as biodegradability and biocompatibility, make them attractive for biomedical applications. Conversely, the major drawback of PLA in the biomedical field is their vulnerability to bacterial contamination. This study focuses on the immobilization of saccharides onto the PLA surface by a multistep approach, with the aim of providing antibacterial features and evaluting the synergistic effect of these saccharides. In this approach, after poly (acrylic acid) (PAA) brushes attached non-covalently to the PLA surface via plasma post-irradiation grafting technique, immobilization of glucosamine (GlcN) and chondroitin sulfate (ChS) to the PAA brushes was carried out. To understand the changes in surface properties, such as chemical composition, surface topography and hydrophilicity, the untreated and treated PLA films were analyzed using various characterization techniques (contact angle, scanning electron microscopy, X-ray photoelectron spectroscopy). In vitro cytotoxicity assays were investigated by the methyl tetrazolium test. The antibacterial activity of the PLA samples was tested against Escherichia coli and Staphylococcus aureus bacteria strains. Plasma-treated films immobilized with ChS and GlcN, separately and in combination, demonstrated bactericidal effect against the both bacteria strains and also the results revealed that the combination has no synergistic effect on antibacterial action. © 2019 by the authors. en
utb.faculty University Institute
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1009027
utb.identifier.obdid 43880563
utb.identifier.scopus 2-s2.0-85070363085
utb.identifier.wok 000480539500103
utb.source j-scopus
dc.date.accessioned 2019-08-21T11:44:09Z
dc.date.available 2019-08-21T11:44:09Z
dc.description.sponsorship Internal Grant Agency of Tomas Bata University in Zlin [IGA/CPS/2019/004]; Czech Science Foundation [17-05095S]; Slovenian Research Agency [L2-8179]
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Karakurt, Ilkay
utb.contributor.internalauthor Özaltin, Kadir
utb.contributor.internalauthor Veselá, Daniela
utb.contributor.internalauthor Lehocký, Marián
utb.contributor.internalauthor Humpolíček, Petr
utb.fulltext.affiliation Ilkay Karakurt 1, Kadir Ozaltin 1, Daniela Vesela 1, Marian Lehocky 1,2*, Petr Humpolíček 1,2, Miran Mozetič 3 1 Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Nam. T.G.M. 5555, 76001 Zlín, Czech Republic 2 Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 76001 Zlín, Czech Republic 3 Department of Surface Engineering, Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia * Correspondence: lehocky@post.cz; Tel.: +420-608-616-048
utb.fulltext.dates Received: 20 June 2019 Accepted: 12 July 2019 Published: 15 July 2019
utb.fulltext.sponsorship The authors thank the Internal Grant Agency of Tomas Bata University in Zlín (IGA/CPS/2019/004) for their financial support, the Czech Science Foundation (17-05095S), and the Slovenian Research Agency (L2-8179).
utb.wos.affiliation [Karakurt, Ilkay; Ozaltin, Kadir; Vesela, Daniela; Lehocky, Marian; Humpolicek, Petr] Tomas Bata Univ Zlin, Ctr Polymer Syst, Univ Inst, Nam TGM 5555, Zlin 76001, Czech Republic; [Lehocky, Marian; Humpolicek, Petr] Tomas Bata Univ Zlin, Fac Technol, Vavreckova 275, Zlin 76001, Czech Republic; [Mozetic, Miran] Jozef Stefan Inst, Dept Surface Engn, Jamova Cesta 39, Ljubljana 1000, Slovenia
utb.scopus.affiliation Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Nam. T.G.M. 5555, Zlín, 76001, Czech Republic; Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, Zlín, 76001, Czech Republic; Department of Surface Engineering, Jozef Stefan Institute, Jamova cesta 39, Ljubljana, 1000, Slovenia
utb.fulltext.projects IGA/CPS/2019/004
utb.fulltext.projects 17-05095S
utb.fulltext.projects L2-8179
utb.fulltext.faculty University Institute
utb.fulltext.faculty University Institute
utb.fulltext.faculty University Institute
utb.fulltext.faculty University Institute
utb.fulltext.faculty Faculty of Technology
utb.fulltext.faculty University Institute
utb.fulltext.faculty Faculty of Technology
utb.fulltext.ou Centre of Polymer Systems
utb.fulltext.ou Centre of Polymer Systems
utb.fulltext.ou Centre of Polymer Systems
utb.fulltext.ou Centre of Polymer Systems
utb.fulltext.ou Centre of Polymer Systems
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International