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Isolation and thermal stabilization of bacteriocin nisin derived from whey for antimicrobial modifications of polymers

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dc.title Isolation and thermal stabilization of bacteriocin nisin derived from whey for antimicrobial modifications of polymers en
dc.contributor.author Holčapková, Pavlína
dc.contributor.author Kolářová Rašková, Zuzana
dc.contributor.author Hrabalíková, Martina
dc.contributor.author Šalaková, Alexandra
dc.contributor.author Drbohlav, Jan
dc.contributor.author Sedlařík, Vladimír
dc.relation.ispartof International Journal of Polymer Science
dc.identifier.issn 1687-9422 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2017
dc.type article
dc.language.iso en
dc.publisher Hindawi Ltd.
dc.identifier.doi 10.1155/2017/3072582
dc.relation.uri https://www.hindawi.com/journals/ijps/2017/3072582/
dc.description.abstract This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80. The stability of samples was tested by means of long-term storage at -18, 4, 25, and 55 degrees C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55 degrees C after 14 days and long-term stability at 25 degrees C with keeping antibacterial activity. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1007663
utb.identifier.obdid 43877131
utb.identifier.scopus 2-s2.0-85042758613
utb.identifier.wok 000415172800001
utb.source j-wok
dc.date.accessioned 2018-01-15T16:31:37Z
dc.date.available 2018-01-15T16:31:37Z
dc.description.sponsorship Ministry of Agriculture of the Czech Republic [QJ1310254]; Ministry of Education, Youth and Sports of the Czech Republic [LO1504]; Internal Grant Agency of Tomas Bata University in Zlin [IGA/CPS/2017/005]
dc.description.sponsorship QJ1310254, MZe, Ministerstvo Zemědělství; LO1504, MEYS, Ministry of Education, Youth and Science; IGA/CPS/2017/005
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Holčapková, Pavlína
utb.contributor.internalauthor Kolářová Rašková, Zuzana
utb.contributor.internalauthor Hrabalíková, Martina
utb.contributor.internalauthor Sedlařík, Vladimír
utb.fulltext.affiliation Ladislav Chaloupský 1, Christopher McKeating 2, Lenka Drábková 3 1 Defense Language Institute, sídl. Víta Nejedlého, 682 03 Vyškov, Czech Republic. Email: vivarep@seznam.cz 2 Defense Language Institute, sídl. Víta Nejedlého, 682 03 Vyškov, Czech Republic. Email: ccm5@st-andrews.ac.uk 3 Tomas Bata University in Zlín, Faculty of Humanities, Department of English and American Studies, Mostní 5139, 760 01 Zlín, Czech Republic. Email: drabkova@fhs.utb.cz
utb.fulltext.dates -
utb.wos.affiliation [Holcapkova, Pavlina; Raskova, Zuzana Kolarova; Hrabalikova, Martina; Sedlarik, Vladimir] Tomas Bata Univ Zlin, Univ Inst, Ctr Polymer Syst, Tr T Bati 5678, Zlin 76001, Czech Republic; [Salakova, Alexandra; Drbohlav, Jan] Dairy Res Inst, Ke Dvoru 12a, Prague 16000 6, Czech Republic
utb.fulltext.faculty Faculty of Humanities
utb.fulltext.ou Department of English and American Studies
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International