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Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1

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dc.title Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1 en
dc.contributor.author Bosák, Juraj
dc.contributor.author Micenková, Lenka
dc.contributor.author Doležalová, Magda
dc.contributor.author Šmajs, David
dc.relation.ispartof International Journal of Medical Microbiology
dc.identifier.issn 1438-4221 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2016
utb.relation.volume 306
utb.relation.issue 7
dc.citation.spage 486
dc.citation.epage 494
dc.type article
dc.language.iso en
dc.publisher Elsevier GmbH - Urban und Fischer
dc.identifier.doi 10.1016/j.ijmm.2016.07.002
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S1438422116301965
dc.subject Escherichia coli en
dc.subject Colicin U en
dc.subject Colicin Y en
dc.subject OmpA en
dc.subject Colicin-receptor interaction en
dc.description.abstract Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpA(Ec)) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpA(SM)) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpA(EC). To verify the importance of the identified amino acid residues in OmpA loop 1, we introduced loop 1 from OmpA(EC) into OmpA(SM), which resulted in the substantial increase of susceptibility to colicins U and Y. In addition, colicins U and Y were tested against a panel of 118 bacteriocin non-producing strains of four Escherichia species, including E. coli (39 strains), E. fergusonii (10 strains), E. hermannii (42 strains), and E. vulneris (27 strains). A majority (82%) of E. coli strains was susceptible to colicins U and Y. Interestingly, colicins U and Y also inhibited all of the 30 tested multidrug-resistant E. coli O25b-ST131 isolates. These findings, together with the fact that OmpA loop 1 is important for bacterial virulence and is evolutionary conserved, offer the potential of using colicins U and Y as specific anti-OmpA loop 1 directed antibacterial proteins. (C) 2016 Elsevier GmbH. All rights reserved. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1006852
utb.identifier.obdid 43875558
utb.identifier.scopus 2-s2.0-84992754260
utb.identifier.wok 000387522200002
utb.identifier.pubmed 27510856
utb.identifier.coden IMEMF
utb.source j-wok
dc.date.accessioned 2017-02-28T15:11:33Z
dc.date.available 2017-02-28T15:11:33Z
dc.description.sponsorship Grant Agency of the Czech Republic [P302/15-18521S, MUNI11/InGA04/2014]
utb.contributor.internalauthor Doležalová, Magda
utb.fulltext.affiliation Juraj Bosák a , Lenka Micenková a , Magda Doležalová b , David Šmajs a,* a Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic b Department of Environment Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, T. G. Masaryk square 275, Zlín, Czech Republic ∗ Corresponding author. E-mail address: dsmajs@med.muni.cz (D. Šmajs).
utb.fulltext.dates Received 25 April 2016 Received in revised form 28 July 2016 Accepted 31 July 2016
utb.fulltext.sponsorship We thank Prof. T. Secrest (Secrest Editing, Ltd.) for the English revision of the manuscript. This work was supported by a grant from the Grant Agency of the Czech Republic (P302/15-18521S to D.S.) and MUNI11/InGA04/2014 (to D.S.).
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