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Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk

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dc.title Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk en
dc.contributor.author Dvořák, Lukáš
dc.contributor.author Mlček, Jiří
dc.contributor.author Šustová, Květoslava
dc.relation.ispartof Journal of AOAC International
dc.identifier.issn 1060-3271 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2016
utb.relation.volume 99
utb.relation.issue 1
dc.citation.spage 180
dc.citation.epage 186
dc.type article
dc.language.iso en
dc.publisher AOAC International
dc.identifier.doi 10.5740/jaoacint.15-0190
dc.relation.uri http://www.ingentaconnect.com/content/aoac/jaoac/2016/00000099/00000001/art00022
dc.description.abstract This study investigated the ability of two methods to detect adulteration of goat cheeses via the addition of cow's milk, with a negligible effect on the raw materials. Cheeses were produced from a mixture of goat's and cow's milk and were then analyzed by Fourier transform near-IR (FT-NIR) spectroscopy and competitive ELISA. The cheese spectra were scanned in the spectroscope in reflectance mode on an integrating sphere at 80 scans and a resolution of 4 cm(-1). The spectra were evaluated via discriminant analysis, and a calibration was created via a partial least-squares algorithm to quantify the cow's milk admixture. A correlation coefficient of R = 0.999 was reached with a standard error of calibration of 0.0407. The results were statistically processed to a median value via a f-test. Adulteration detection by the ELISA method was performed using a commercial Milk Fraud/Bovine ELISA kit. It was found that the FT-NIR spectroscopy method is capable of detecting an admixture of cow's milk in goat cheese as small as 1%. The ELISA method did not return satisfactory results for the detection of adulteration with cow's milk. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1006347
utb.identifier.obdid 43875136
utb.identifier.scopus 2-s2.0-84962374828
utb.identifier.wok 000371953400022
utb.identifier.pubmed 26822518
utb.identifier.coden JAINE
utb.source j-wok
dc.date.accessioned 2016-06-22T12:14:50Z
dc.date.available 2016-06-22T12:14:50Z
dc.description.sponsorship NAZV [KUSQJ1230044]; Tomas Bata University in Zlin [IGA/FT/2016/008]
utb.contributor.internalauthor Mlček, Jiří
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