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Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

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dc.title Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies en
dc.contributor.author Bober, Patrycja
dc.contributor.author Humpolíček, Petr
dc.contributor.author Pacherník, Jiří
dc.contributor.author Stejskal, Jaroslav
dc.contributor.author Lindfors, Tom
dc.relation.ispartof RSC Advances
dc.identifier.issn 2046-2069 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2015
utb.relation.volume 5
utb.relation.issue 62
dc.citation.spage 50328
dc.citation.epage 50335
dc.type article
dc.language.iso en
dc.publisher Royal Society of Chemistry (RSC)
dc.identifier.doi 10.1039/c5ra07504a
dc.relation.uri http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA07504A#!divAbstract
dc.description.abstract In this work, thin films consisting of electrically conducting polyaniline (PANI) and poly(2-acrylamido-2-methyl-1-propanesulfonate) (PAMPSA) have been used as cell culture substrates for embryonic stem cells (ESC) and embryoid bodies (EMB). The PANI-PAMPSA films having fibrillar morphology were electrochemically polymerized in a single-step by cyclic voltammetry from an aqueous solution containing aniline and PAMPSA. UV-visible spectroscopy showed that the PANI films were electrically conducting still at pH 10. This makes them suitable for tissue engineering applications operating at physiological pH, in contrast to the commonly used PANI hydrochloride films which loose their electrical conductivity at pH >= 4. Our results reveal that the PANI-PAMPSA films allow only for limited ESC adhesion and growth. The inhibition of the EMB growth and adhesion on the PANI-PAMPSA surface in serum-free medium indicates that it can be used as a cell-selective substrate for the growth of only some specific differentiated EMB cell types. en
utb.faculty Faculty of Technology
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1005227
utb.identifier.rivid RIV/70883521:28610/15:43872986!RIV16-GA0-28610___
utb.identifier.obdid 43873406
utb.identifier.scopus 2-s2.0-84935864093
utb.identifier.wok 000355987900065
utb.identifier.coden RSCAC
utb.source j-wok
dc.date.accessioned 2015-07-24T08:15:53Z
dc.date.available 2015-07-24T08:15:53Z
dc.description.sponsorship Johan Gadolin Scholarship Programme within Johan Gadolin Process Chemistry Centre at Abo Akademi University (Finland); Academy of Finland [130588, 263656]; Czech Science Foundation [13-08944S]
dc.description.sponsorship 13-08944S, GACR, Czech Science Foundation
dc.rights Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.access openAccess
utb.contributor.internalauthor Humpolíček, Petr
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Attribution-NonCommercial-NoDerivs 3.0 Unported Kromě případů, kde je uvedeno jinak, licence tohoto záznamu je Attribution-NonCommercial-NoDerivs 3.0 Unported