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Selected factors influencing the ability of Bifidobacterium to form biogenic amines

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dc.title Selected factors influencing the ability of Bifidobacterium to form biogenic amines en
dc.contributor.author Lorencová, Eva
dc.contributor.author Buňková, Leona
dc.contributor.author Pleva, Pavel
dc.contributor.author Dráb, Vladimír
dc.contributor.author Kubáň, Vlastimil
dc.contributor.author Buňka, František
dc.relation.ispartof International Journal of Food Science and Technology
dc.identifier.issn 0950-5423 Scopus Sources, Sherpa/RoMEO, JCR
dc.identifier.issn 1365-2621 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2014
utb.relation.volume 49
utb.relation.issue 5
dc.citation.spage 1302
dc.citation.epage 1307
dc.type article
dc.language.iso en
dc.publisher Wiley-Blackwell Publishing Ltd.
dc.identifier.doi 10.1111/ijfs.12427
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1111/ijfs.12427/abstract
dc.subject tyramine en
dc.subject Bifidobacterium en
dc.subject biogenic amines en
dc.subject external factors en
dc.description.abstract The ability of typical probiotic culture of Bifidobacterium to produce biogenic amines could be considered a contrastive feature to the beneficial dietary effect on human health. The aim of this pilot study was to evaluate the decarboxylase activity of Bifidobacterium animalis subsp. lactis CCDM 239 influenced by selected factors (pH 4.5 and 5.0; the contents of NaCl 0-20.0 g L-1, glucose and lactose 0-10.0 g L-1) at in vitro conditions. The kinetics of the biogenic amine production under the above-mentioned conditions was also monitored. The biogenic amine content detection was carried out in the supernatants of inoculated broth [MRS enriched with amino acids: arginine, ornithine, lysine, tyrosine; 3 g L-1 after the cultivation (48 h, 37 ± 1 °C)]. RP-HPLC after the precolumn derivatisation with dansyl chloride was used. In most cases, the low concentrations of tyramine were monitored (<15 mg L-1). Simultaneously, it was found out that the addition of certain fermentable saccharide concentrations and NaCl in their mutual combination seemed to have supporting effect on the decarboxylase activity of the tested Bifidobacterium. © 2013 Institute of Food Science and Technology. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1003735
utb.identifier.obdid 43871958
utb.identifier.scopus 2-s2.0-84898428151
utb.identifier.wok 000334079700009
utb.source j-scopus
dc.date.accessioned 2014-06-05T20:22:54Z
dc.date.available 2014-06-05T20:22:54Z
utb.contributor.internalauthor Lorencová, Eva
utb.contributor.internalauthor Buňková, Leona
utb.contributor.internalauthor Pleva, Pavel
utb.contributor.internalauthor Kubáň, Vlastimil
utb.contributor.internalauthor Buňka, František
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