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Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito

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dc.title Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito en
dc.contributor.author Šikutová, Silvie
dc.contributor.author Buňková, Leona
dc.contributor.author Krejčí, Eva
dc.contributor.author Halouzka, Jiří
dc.contributor.author Sanogo, Yibayiri O.
dc.contributor.author Rudolf, Ivo
dc.relation.ispartof Microbiological Research
dc.identifier.issn 0944-5013 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2014
utb.relation.volume 169
utb.relation.issue 5-6
dc.citation.spage 348
dc.citation.epage 352
dc.type article
dc.language.iso en
dc.publisher Urban & Fischer Verlag
dc.identifier.doi 10.1016/j.micres.2013.10.002
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0944501313001717
dc.subject Culex pipiens en
dc.subject Enzyme activity en
dc.subject FAME en
dc.subject SDS-PAGE en
dc.subject Spirochaete en
dc.description.abstract Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33°C. Strain BR91 was sensitive to trimethoprim, nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and α-fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed several major proteins in the size range of 13-16kDa, 22-30kDa and 37-131kDa. Fatty acid methyl ester (FAME) analysis showed that C14:0, C16:0, C18:1 ω9c and summed feature 5 (C18:2 ω6,9c and/or C18:0 anteiso) are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and the Lyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents a unique taxonomical entity in a system of spirochaetal species. © 2013 Elsevier GmbH. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1003718
utb.identifier.obdid 43871784
utb.identifier.scopus 2-s2.0-84896545445
utb.identifier.wok 000335107900003
utb.identifier.coden MCRSE
utb.source j-scopus
dc.date.accessioned 2014-05-07T13:49:23Z
dc.date.available 2014-05-07T13:49:23Z
dc.description.sponsorship Czech Republic [206/00/1234, 206/03/0726]; Ministry of Education of the Czech Republic [FRVS 544/2003]; Ministry of Health [MZO/ZA/00538]; FEMS Congress of European Microbiologists, Ljubljana, Slovenia; Operational Programme Education for Competitiveness [CZ.1.07/2.3.00/20.0183]
utb.contributor.internalauthor Buňková, Leona
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