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Vývoj metodiky v diagnostice heparinem indukované trombocytopenie

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dc.title Vývoj metodiky v diagnostice heparinem indukované trombocytopenie cs
dc.title The development of methods in the diagnostics of heparin-induced trombocytopenia en
dc.contributor.author Andělová, Kateřina
dc.contributor.author Minář, Jan
dc.contributor.author Králová, Soňa
dc.contributor.author Hrabcová, R.
dc.contributor.author Radina, Martin
dc.contributor.author Kučerová, Miroslava
dc.relation.ispartof Klinická biochemie a metabolismus
dc.identifier.issn 1210-7921 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2007
utb.relation.volume 15
utb.relation.issue 4
dc.citation.spage 215
dc.citation.epage 217
dc.type article
dc.language.iso cs
dc.publisher Česká lékařská společnost Jana Evangelisty Purkyně (ČLS JEP)
dc.relation.uri http://www.cskb.cz/res/file/KBM-pdf/2007/4-07/KBM_04_07_Andelova_215.pdf
dc.subject Diagnostics en
dc.subject Heparin induced thrombocytopenia en
dc.subject HPLC en
dc.subject Serotonin release en
dc.description.abstract Objective: Heparin-induced thrombocytopenia (HIT II) is an immune-mediated disorder observed in up to 0.5-5% of heparin-treated patients. Laboratory testing for the diagnosis of HIT II includes a 14C-serotonin release test, PF4/heparin enzyme-immunoassay, platelet aggregation and flow cytometric assay with various specificities or sensitivities for the diagnosis of HIT II. The aim of this work was to develop an HPLC method with electrochemical detection (EC) and prove the possibility of using a non-radioactive serotonin release test for the diagnosis of HIT II. Material and Methods: Two groups were tested. First group of patients suspect for HIT II, and second control group with or without heparin therapy. Serotonin release was analyzed by a newly developed HPLC-EC method. Results: Analytical performance of this method was satisfactory. The method was linear from 5 to 7000 nmol/l with a limit of detection of 1 nmol/l. The intra- and inter-assay coefficients of variation were 7.7% and 12%, respectively, for a concentration of serotonin 1460 nmol/l. Conclusion: In the present work we describe rapid, non-radioactive, specific and sensitive HPLC-ED methods to determine serotonin release from the donor platelets. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1003704
utb.identifier.scopus 2-s2.0-78649970237
utb.identifier.coden KBMEF
utb.source j-scopus
dc.date.accessioned 2014-04-16T13:35:43Z
dc.date.available 2014-04-16T13:35:43Z
utb.contributor.internalauthor Minář, Jan
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