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Different source atelocollagen thin films: Preparation, process optimisation and its influence on the interaction with eukaryotic cells

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dc.title Different source atelocollagen thin films: Preparation, process optimisation and its influence on the interaction with eukaryotic cells en
dc.contributor.author López García, Jorge Andrés
dc.contributor.author Humpolíček, Petr
dc.contributor.author Lehocký, Marián
dc.contributor.author Junkar, Ita
dc.contributor.author Mozetič, Miran
dc.relation.ispartof Materiali in Tehnologije
dc.identifier.issn 1580-2949 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2013
utb.relation.volume 47
utb.relation.issue 4
dc.citation.spage 473
dc.citation.epage 479
dc.type article
dc.language.iso en
dc.publisher Institut za Kovinske Materiale in Tehnologije Ljubljana sl
dc.relation.uri http://mit.imt.si/Revija/mit134.html
dc.subject Atelocollagen thin films en
dc.subject Eukaryotic cell response en
dc.subject Film optimisation en
dc.subject Film quality en
dc.subject Surface topography en
dc.description.abstract Collagen thin films were prepared via bovine atelocollagen matrices. The film casting was carried out by using different culture dishes, concentrations, equipment, drying processes and periods of time. In order to optimise the repeatability and reproducibility, microscopic analyses were utilised to explore the film quality and topographical patterning. In addition, the human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded onto the obtained specimens, and the cell proliferation was determined by using the MTT assay. These results indicated how the substrate, its concentration and processing conditions influence the cellular response. The attempted technique shows itself to be an excellent procedure for continuous collagen film preparation with optimal cell-proliferation rates, which may potentially be used in tissue engineering or wound-healing applications. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1003443
utb.identifier.obdid 43869926
utb.identifier.scopus 2-s2.0-84881091292
utb.identifier.wok 000323085200013
utb.source j-scopus
dc.date.accessioned 2013-08-21T10:18:13Z
dc.date.available 2013-08-21T10:18:13Z
dc.rights.uri http://mit.imt.si/
dc.rights.access openAccess
utb.contributor.internalauthor López García, Jorge Andrés
utb.contributor.internalauthor Humpolíček, Petr
utb.contributor.internalauthor Lehocký, Marián
utb.fulltext.affiliation Jorge López García1, Petr Humpolíček1, Marián Lehocký1, Ita Junkar2, Miran Mozetič2 1 Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic 2 Department of Surface Engineering, Plasma Laboratory, Jo`ef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia lehocky@post.cz
utb.fulltext.dates Prejem rokopisa – received: 2012-11-16; sprejem za objavo – accepted for publication: 2013-01-09
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utb.fulltext.sponsorship The authors would like to express their gratitude to the Ministry of Education, Youth and Sport of the Czech Republic (CZ.1.05/2.1.00/03.0111). The Slovenia Ministry of Higher Education, Science, and Technology (Program P2-0082-2) and Ad Futura L7-4009 are also gratefully acknowledged for the financial support of this research.
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