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Decarboxylation activity of enterococci isolated from rabbit meat and staphylococci isolated from trout intestines

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dc.title Decarboxylation activity of enterococci isolated from rabbit meat and staphylococci isolated from trout intestines en
dc.contributor.author Pleva, Pavel
dc.contributor.author Buňková, Leona
dc.contributor.author Lauková, Andrea
dc.contributor.author Lorencová, Eva
dc.contributor.author Kubáň, Vlastimil
dc.contributor.author Buňka, František
dc.relation.ispartof Veterinary Microbiology
dc.identifier.issn 0378-1135 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2012
utb.relation.volume 159
utb.relation.issue 3-4
dc.citation.spage 438
dc.citation.epage 442
dc.type article
dc.language.iso en
dc.publisher Elsevier Science B.V. en
dc.identifier.doi 10.1016/j.vetmic.2012.04.028
dc.subject Biogenic amines en
dc.subject Enterococcus en
dc.subject Food safety en
dc.subject High performance liquid chromatography en
dc.subject Staphylococcus en
dc.description.abstract The aim of the study was to explore production of seven biogenic amines (phenylethylamine, histamine, cadaverine, tyramine, putrescine, spermine and/or spermidine) by selected staphylococci and enterococci. Thirty three enterococcal strains isolated from rabbit meat (Oryctolagus cuniculus f. domesticus) and 21 staphylococcal strains isolated from intestinal content of trout (Salmo trutta morpha fario) were tested. Production of biogenic amines was evaluated after cultivation of the tested microorganisms in the de Man, Rogosa and Sharpe Broth (enterococci) or in the Brain Heart Infusion Broth (staphylococci). Both the above cultivation media were enriched with selected amino acids (histidine, tyrosine, arginine, ornithine and lysine; 2. g/L each) serving as precursors of biogenic amines. After cultivation, levels of the monitored biogenic amines in broths were analysed by a high performance liquid chromatography equipped with a UV/VIS DAD detector. Among 21 staphylococci, 18 strains produced tyramine or cadaverine, 13 strains formed putrescine or phenylethylamine and only one strain generated histamine. Two staphylococcal strains produced cadaverine levels above 1000. mg/L. Among 33 enterococcal strains, 27 formed cadaverine, 18 strains produced tyramine, 10 strains generated phenylethylamine, and 2 strains gave putrescine. Most of the tyramine producing enterococci generated more than 1000. mg/L of this biogenic amine. Production of spermine or spermidine by the studied strains was not proved. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1002930
utb.identifier.rivid RIV/70883521:28110/12:43867958!RIV13-MSM-28110___
utb.identifier.obdid 43868049
utb.identifier.scopus 2-s2.0-84865647241
utb.identifier.wok 000309316000022
utb.identifier.coden VMICD
utb.source j-scopus
dc.date.accessioned 2012-09-17T16:22:14Z
dc.date.available 2012-09-17T16:22:14Z
utb.contributor.internalauthor Pleva, Pavel
utb.contributor.internalauthor Buňková, Leona
utb.contributor.internalauthor Lorencová, Eva
utb.contributor.internalauthor Kubáň, Vlastimil
utb.contributor.internalauthor Buňka, František
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