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Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment

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dc.title Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment en
dc.contributor.author López García, Jorge Andrés
dc.contributor.author Pacherník, Jiří
dc.contributor.author Lehocký, Marián
dc.contributor.author Junkar, Ita
dc.contributor.author Humpolíček, Petr
dc.contributor.author Sáha, Petr
dc.relation.ispartof Progress in Colloid and Polymer Science
dc.identifier.issn 0340-255X Scopus Sources, Sherpa/RoMEO, JCR
dc.identifier.isbn 9783642190377
dc.date.issued 2010
utb.relation.volume 138
utb.relation.issue 2011
dc.citation.spage 89
dc.citation.epage 94
dc.type article
dc.language.iso en
dc.publisher Springer-Verlag Berlin en
dc.identifier.doi 10.1007/978-3-642-19038-4_16
dc.relation.uri http://www.springerlink.com/content/p62786857866589h/
dc.subject Atelocollagen en
dc.subject Cell adhesion en
dc.subject Cell growth en
dc.subject Plasma treatment en
dc.subject Radio frequency discharge en
dc.subject Thin films en
dc.description.abstract Collagen films (Atelocollagen from bovine Achilles tendon) were prepared in tissue culture dishes and their surfaces were modified by using air and nitrogen plasma treatment. The treated samples were characterised by surface probe techniques including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy imaging (SEM) and X-ray photoelectron spectroscopy (XPS). In addition, human immortalised nontumorigenic keratinocyte cell line (HaCaT) was seeded on the treated and untreated films and cell proliferation was measured by using MTT assay test. The characterisation results confirmed physical and chemical changes on the collagen surface, such as increase in the extent of surface oxidation and surface roughness as well as, the treated samples showed better cell growth than untreated ones, and therefore this approach may be taken into account in the development of promising materials for tissue regeneration applications. © Springer-Verlag Berlin Heidelberg 2011. en
utb.faculty Faculty of Technology
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1002688
utb.identifier.rivid RIV/70883521:28110/11:43865491!RIV12-MSM-28110___
utb.identifier.rivid RIV/70883521:28610/11:43865491!RIV12-MSM-28610___
utb.identifier.obdid 43865502
utb.identifier.scopus 2-s2.0-80051609642
utb.identifier.wok 000295091800016
utb.identifier.coden PCPSD
utb.source j-scopus
dc.date.accessioned 2012-02-10T13:15:31Z
dc.date.available 2012-02-10T13:15:31Z
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor López García, Jorge Andrés
utb.contributor.internalauthor Lehocký, Marián
utb.contributor.internalauthor Humpolíček, Petr
utb.contributor.internalauthor Sáha, Petr
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