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Determination of vitamin C (ascorbic acid) using high performance liquid chromatography coupled with electrochemical detection

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dc.title Determination of vitamin C (ascorbic acid) using high performance liquid chromatography coupled with electrochemical detection en
dc.contributor.author Gazdík, Zbyněk
dc.contributor.author Zítka, Ondřej
dc.contributor.author Petrlová, Jitka
dc.contributor.author Adam, Vojtěch
dc.contributor.author Zehnálek, Josef
dc.contributor.author Horna, Aleš
dc.contributor.author Řezníček, Vojtěch
dc.contributor.author Beklová, Miroslava
dc.contributor.author Kizek, René
dc.relation.ispartof Sensors
dc.identifier.issn 1424-8220 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2008-11
utb.relation.volume 8
utb.relation.issue 11
dc.citation.spage 7097
dc.citation.epage 7112
dc.type article
dc.language.iso en
dc.publisher Molecular Diversity Preservation International (MDPI) en
dc.identifier.doi 10.3390/s8117097
dc.relation.uri http://www.mdpi.com/1424-8220/8/11/7097/
dc.subject Ascorbic Acid en
dc.subject Flow Injection Analysis en
dc.subject High Performance Liquid Chromatography en
dc.subject Electrochemical Detection en
dc.subject Fruits en
dc.subject Pharmaceutical Preparation en
dc.subject Human Blood Serum en
dc.description.abstract Vitamin C (ascorbic acid, ascorbate, AA) is a water soluble organic compound that participates in many biological processes. The main aim of this paper was to utilize two electrochemical detectors (amperometric - Coulouchem III and coulometric - CoulArray) coupled with flow injection analysis for the detection of ascorbic acid. Primarily, we optimized the experimental conditions. The optimized conditions were as follows: detector potential 100 mV, temperature 25 C, mobile phase 0.09% TFA: ACN, 3: 97 (v/v) and flow rate 0.13 mL.min(-1). The tangents of the calibration curves were 0.3788 for the coulometric method and 0.0136 for the amperometric one. The tangent of the calibration curve measured by the coulometric detector was almost 30 times higher than the tangent measured by the amperometric detector. Consequently, we coupled a CoulArray electrochemical detector with high performance liquid chromatography and estimated the detection limit for AA as 90 nM (450 fmol per 5 mu L injection). The method was used for the determination of vitamin C in a pharmaceutical preparations ( 98 +/- 2 mg per tablet), in oranges ( Citrus aurantium) ( varied from 30 to 56 mg/100 g fresh weight), in apples (Malus sp.) (varied from 11 to 19 mg/100 g fresh weight), and in human blood serum (varied from 38 to 78 mu M). The recoveries were also determined. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1002134
utb.identifier.rivid RIV/70883521:28110/08:43865378!RIV12-MSM-28110___
utb.identifier.obdid 43865382
utb.identifier.scopus 2-s2.0-56449105282
utb.identifier.wok 000261215000018
utb.source j-wok
dc.date.accessioned 2011-08-16T15:06:32Z
dc.date.available 2011-08-16T15:06:32Z
dc.rights Attribution 3.0 International
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.rights.access openAccess
utb.contributor.internalauthor Horna, Aleš
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