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A sensor for investigating the interaction between biologically important heavy metals and glutathione

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dc.title A sensor for investigating the interaction between biologically important heavy metals and glutathione en
dc.contributor.author Húska, Dalibor
dc.contributor.author Zítka, Ondřej
dc.contributor.author Adam, Vojtěch
dc.contributor.author Beklová, Miroslava
dc.contributor.author Křížková, Soňa
dc.contributor.author Zeman, Ladislav
dc.contributor.author Horna, Aleš
dc.contributor.author Havel, Ladislav
dc.contributor.author Zehnálek, Josef
dc.contributor.author Kizek, René
dc.relation.ispartof Czech Journal of Animal Science
dc.identifier.issn 1212-1819 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2007-02
utb.relation.volume 52
utb.relation.issue 2
dc.citation.spage 37
dc.citation.epage 43
dc.type article
dc.language.iso en
dc.publisher National Institute of Food and Agriculture (NIFA) en
dc.identifier.doi 10.17221/2345-CJAS
dc.relation.uri https://www.agriculturejournals.cz/web/cjas.htm?volume=52&firstPage=37&type=publishedArticle
dc.subject reduced glutathione (GSH) en
dc.subject oxidized glutathione (GSSG) en
dc.subject thiols en
dc.subject cadmium en
dc.subject zinc en
dc.subject copper en
dc.subject nickel en
dc.subject additive en
dc.subject cyclic voltammetry en
dc.subject electrochemical method en
dc.description.abstract Glutathione (GSH) is a water-soluble tripeptide playing a crucial role in the number of cellular reactions including detoxification of heavy metals. Glutathione can be found both in an oxidized (GSSG) and reduced (GSH) state. The aim of this paper was to use electrochemical methods to determine glutathiones and to investigate interactions of GSH and cadmium, zinc, copper and nickel ions or feed additive for animal nutrition. It clearly follows from the results that GSH and GSSG can be distinguished by cyclic voltammetry whereas the level of their current response is proportional to their concentration. Moreover, we studied these interactions by cyclic voltammetry. Marked changes in the GSH signal were observed and discussed. The highest decrease in a GSH reductive signal is caused by cadmium ions followed by zinc, nickel and copper ones. We also observed that the reductive GSH signal gradually decreased (about 9%) with the increasing concentration of feed additive used in our experiments. In conclusion, cyclic voltammetry offers a tool for studying interactions of thiols with different substances such as heavy metals and/or feed additives. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1002091
utb.identifier.obdid 43865415
utb.identifier.scopus 2-s2.0-33847327905
utb.identifier.wok 000244285800002
utb.source j-wok
dc.date.accessioned 2011-08-16T15:06:27Z
dc.date.available 2011-08-16T15:06:27Z
utb.contributor.internalauthor Horna, Aleš
utb.fulltext.affiliation D. Húska1,2, O. Zítka2,3, V. Adam2,4, M. Beklová5, S. Křížková2,4, L. Zeman6, A. Horna7, L. Havel1, J. Zehnálek2, R. Kizek2 1 Department of Plant Biology, Mendel University of Agriculture and Forestry, Brno, Czech Republic 2 Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic 6 Department of Animal Nutrition, Mendel University of Agriculture and Forestry, Brno, Czech Republic 3 Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic 4 Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic 5 Department of Veterinary Ecology and Environment Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 7 Department of Food Engineering and Chemistry, Faculty of Technology, Tomas Bata University, Zlín, Czech Republic Corresponding Author Doc. Ing. René Kizek, PhD., Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, 613 00 Brno, Czech Republic Tel. +420 545 133 350, fax +420 545 212 044, e-mail: kizek@sci.muni.cz
utb.fulltext.dates Received: 2006–09–06 Accepted after corrections: 2006–12–07
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utb.fulltext.sponsorship Supported by the Ministry of Education, Youth and Sports of the Czech Republic (Project No. MSMT 6215712402) and Czech Science Foundation (Grant No. 525/04/P132).
utb.fulltext.projects MSM 6215712402
utb.fulltext.projects GACR 525/04/P132
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