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Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes

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dc.title Development of a rapid, simple paddle-style dipstick dye immunoassay specific for Listeria monocytogenes en
dc.contributor.author Blažková, Martina
dc.contributor.author Karamonová, Ludmila
dc.contributor.author Greifová, Mária
dc.contributor.author Fukal, Ladislav
dc.contributor.author Hoza, Ignác
dc.contributor.author Rauch, Pavel
dc.contributor.author Wyatt, Gary
dc.relation.ispartof European Food Research and Technology
dc.identifier.issn 1438-2377 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2006-10
utb.relation.volume 223
utb.relation.issue 6
dc.citation.spage 821
dc.citation.epage 827
dc.type article
dc.language.iso en
dc.publisher Springer en
dc.identifier.doi 10.1007/s00217-006-0275-3
dc.relation.uri http://www.springerlink.com/content/j124381h26r57026/
dc.subject dipstick immunoassay en
dc.subject Listeria monocytogenes en
dc.subject milk en
dc.subject ice cream en
dc.description.abstract We have developed two types of new paddle-style dipstick dye immunoassays. The first is genus Listeria specific and the second is specific to Listeria monocytogenes. They are based respectively, on antisera raised against heat-killed L. monocytogenes cells and against internalin B crude extract, a virulence protein found only in the pathogenic L. monocytogenes. The minimum detectable level for L. monocytogenes is 2x10(7) CFU ml(-1) for strain number 88/049 in pure culture. Detection is unaffected by the presence of high numbers (approximately log 8.0 CFU/ml) of the other microorganisms tested. When the dipsticks were applied to milk samples inoculated with L. monocytogenes reference material (ALM92), there was a strong response to the enrichment cultures. The new assay may prove useful in detection of L. monocytogenes in enrichment cultures of milk and ice cream food samples. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1002084
utb.identifier.obdid 43865422
utb.identifier.scopus 2-s2.0-33749019468
utb.identifier.wok 000240718400018
utb.source j-wok
dc.date.accessioned 2011-08-16T15:06:26Z
dc.date.available 2011-08-16T15:06:26Z
utb.contributor.internalauthor Hoza, Ignác
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