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Bioactive phenols in algae: The application of pressurized-liquid and solid-phase extraction techniques

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dc.title Bioactive phenols in algae: The application of pressurized-liquid and solid-phase extraction techniques en
dc.contributor.author Onofrejová, Lucia
dc.contributor.author Vašíčková, Jana
dc.contributor.author Klejdus, Bořivoj
dc.contributor.author Stratil, Pavel
dc.contributor.author Mišurcová, Ladislava
dc.contributor.author Kráčmar, Stanislav
dc.contributor.author Kopecký, Jan
dc.contributor.author Vacek, Jan
dc.relation.ispartof Journal of Pharmaceutical and Biomedicinal Analysis
dc.identifier.issn 0731-7085 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2010
utb.relation.volume 51
utb.relation.issue 1
dc.citation.spage 464
dc.citation.epage 470
dc.type article
dc.language.iso en
dc.publisher Elsevier Science B.V. en
dc.identifier.doi 10.1016/j.jpba.2009.03.027
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0731708509002088
dc.subject phenols and aldehydes en
dc.subject algae en
dc.subject antioxidants en
dc.subject benzoic and cinnamic acid derivatives en
dc.description.abstract Nová extrakční metoda založená na kombinaci extrakce kapalina-tuhá fáze (PLE-SPE) byla použita pro extrakci bioaktivních fenolických látek z in vitro kultur dvou sladkovodních řas (Anabaena doliolum and Spongiochloris spongiosa) a z potravních produktů z mořských řas Porphyra tenera (nori) a Undaria pinnatifida (wakame). Analýzy prokázaly, že ve sladkovodních řasách a produktech z mořských řas byly fenolické látky obsaženy v hodnotách mikrogramů na gram lyofylizovaného vzorku. Dále byl stanoven celkový obsah fenolických látek (Folin?Ciocalteu metoda) a antioxidační aktivita (TEAC metoda) PLE-SPE extraktů. cs
dc.description.abstract A new extraction technique based on the off-line combination of pressurized-liquid with solid-phase extraction (PLE?SPE) is described. Themethodwas used for the extraction of bioactive phenolic acids (protocatechuic, p-hydroxybenzoic, 2,3-dihydroxybenzoic, chlorogenic, vanillic, caffeic, p-coumaric, salicylic acid), cinnamic acid and hydroxybenzaldehydes (p-hydroxybenzaldehyde, 3,4-dihydroxybenzaldehyde, vanillin) from in vitro culture of two freshwater algae (Anabaena doliolum and Spongiochloris spongiosa) and fromfood products of marine macroalgae Porphyra tenera (nori) andUndaria pinnatifida(wakame). For the identification and quantification of the compounds the molecular ions [M?H]? and specific fragments were analyzed by quadrupole mass spectrometry analyzer connected on-line with a reversed-phase HPLC system. Our analysis showed that the freshwater algae and marine algal products contained submicrogram or microgram level of above-mentioned phenols per gram of lyophilized sample. In addition, the total phenol content (Folin?Ciocalteu assay) and antioxidant activity (TEAC assay, Trolox equivalent antioxidant capacity assay) of the PLE?SPE extracts were determined and discussed. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1001212
utb.identifier.rivid RIV/70883521:28110/10:63509519!RIV11-MSM-28110___
utb.identifier.obdid 43864743
utb.identifier.scopus 2-s2.0-70349841332
utb.identifier.wok 000271309700018
utb.source j-riv
utb.contributor.internalauthor Mišurcová, Ladislava
utb.contributor.internalauthor Kráčmar, Stanislav
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