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Study of changes in unripened soft rennet-curd cheeses caused by the addition of dried nettle leaves: Physicochemical properties, microbial quality, polyphenol content, and sensory characteristics

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dc.title Study of changes in unripened soft rennet-curd cheeses caused by the addition of dried nettle leaves: Physicochemical properties, microbial quality, polyphenol content, and sensory characteristics en
dc.contributor.author Pluta-Kubica, Agnieszka
dc.contributor.author Najgebauer-Lejko, Dorota
dc.contributor.author Pająk, Paulina Maria
dc.contributor.author Sobolewska-Zielińska, Joanna
dc.contributor.author Skoczylas, Łukasz
dc.contributor.author Aththota Gamage, A. C.K.
dc.contributor.author Kratochvílová, Alena
dc.contributor.author Salek, Richardos-Nicolaos
dc.contributor.author Duda, Iwona
dc.contributor.author Domagała, Jacek
dc.relation.ispartof Journal of Dairy Science
dc.identifier.issn 0022-0302 Scopus Sources, Sherpa/RoMEO, JCR
dc.identifier.issn 1525-3198 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2026
utb.relation.volume 109
utb.relation.issue 2
dc.citation.spage 1082
dc.citation.epage 1095
dc.type article
dc.language.iso en
dc.publisher Elsevier Inc.
dc.identifier.doi 10.3168/jds.2025-27260
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0022030225009981?via%3Dihub
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0022030225009981/pdfft?md5=c5295754b55eecec6f111dad9fe37fa4&pid=1-s2.0-S0022030225009981-main.pdf
dc.subject cheese en
dc.subject nettle en
dc.subject microbiology en
dc.subject polyphenols en
dc.subject sensory analysis en
dc.description.abstract The objectives of the study were to investigate changes in the number of selected groups of microorganisms and the level of antioxidant activity during storage of model unripened cheeses with the addition of dried nettle leaves, to compare their sensory characteristics, as well as to determine the antioxidant properties of the obtained whey. Three groups of model unripened rennet-curd cheeses were produced: I—a control sample without a plant additive, II and III—samples with the addition of dried nettle leaves in the amount of 0.165% and 0.330% (g/100 g of milk), respectively. Advantageously, no effects of nettle addition on total microbial and Lactococcus counts were determined. Moreover, the application of this herb slowed down the yeast and mold growth in cheese, increased the antioxidant activity, and elevated the contents of total polyphenols, caffeic acid, p-coumaric acid, rutin, and ferulic acid. Additionally, the antioxidant properties of the obtained whey were also higher in comparison to control. Sensory analysis revealed that the addition of a lower amount (0.165%) of dried nettle leaves appeared to be the most favorable option, balancing improved nutritional and antioxidant value with acceptable sensory characteristics and acceptability. The study demonstrated that the application of dried nettle leaves as an addition to cheese offers many benefits. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1012796
utb.identifier.scopus 2-s2.0-105028597311
utb.identifier.wok 001680824700003
utb.identifier.pubmed 41349825
utb.source j-scopus
dc.date.accessioned 2026-04-30T12:07:57Z
dc.date.available 2026-04-30T12:07:57Z
dc.description.sponsorship Funding text 1: The analyses of the physicochemical properties of cheese and whey, microbiological quality of cheese, as well as antioxidant activity and total content of polyphenols in cheese and whey were financed by the National Science Centre, Poland\u2013MINIATURA 7, grant no. 2023/07/X/NZ9/00618. The analyses of the polyphenolic profile in nettle leaves, whey, and cheese as well as the sensory quality of cheese were supported by funds from the project: the task titled \u201CResearch network of life science universities for the development of the Polish dairy sector\u2014Research project\u201D financed under the targeted subsidy of the Minister of Science and Higher Education (Warsaw, Poland; no. MEiN/2023/DPI/2862). For publication, the funds from the project \u201CResearch network of life science universities for the development of the Polish dairy sector\u2014Research project\u201D financed under the targeted subsidy of the Minister of Science and Higher Education (Warsaw, Poland; no. MEiN/2023/DPI/2862) were used. The research activities regarding sensory analysis were approved by the University of Agriculture Ethics Committee (approval no. 189/2024, May 23, 2024, and approval no. 316/2025, September 29, 2025), and all participants provided informed consent. The authors have not stated any conflicts of interest.; Funding text 2: The analyses of the physicochemical properties of cheese and whey, microbiological quality of cheese, as well as antioxidant activity and total content of polyphenols in cheese and whey were financed by the National Science Centre, Poland\u2013MINIATURA 7, grant no. 2023/07/X/NZ9/00618. The analyses of the polyphenolic profile in nettle leaves, whey, and cheese as well as the sensory quality of cheese were supported by funds from the project: the task titled \u201CResearch network of life science universities for the development of the Polish dairy sector\u2014Research project\u201D financed under the targeted subsidy of the Minister of Science and Higher Education (Warsaw, Poland; no. MEiN/2023/DPI/2862). For publication, the funds from the project \u201CResearch network of life science universities for the development of the Polish dairy sector\u2014Research project\u201D financed under the targeted subsidy of the Minister of Science and Higher Education (Warsaw, Poland; no. MEiN/2023/DPI/2862) were used. The research activities regarding sensory analysis were approved by the University of Agriculture Ethics Committee (approval no. 189/2024, May 23, 2024, and approval no. 316/2025, September 29, 2025), and all participants provided informed consent. The authors have not stated any conflicts of interest. Nonstandard abbreviations used: ARP = antiradical power; DPPH = 2,2-diphenyl-1-picrylhydrazyl; EC50 = amount of sample required to decrease the DPPH radical concentration by 50%; GAE = gallic acid equivalent; LAB = lactic acid bacteria; QDA = quantitative descriptive analysis; TE = Trolox equivalent; TPC = total phenolic content; TVC = total viable count.
dc.description.sponsorship National Science Centre, Poland-MINIATURA 7 [2023/07/X/NZ9/00618]; Minister of Science and Higher Education [MEiN/2023/DPI/2862]
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.contributor.internalauthor Kratochvílová, Alena
utb.contributor.internalauthor Salek, Richardos-Nicolaos
utb.fulltext.sponsorship The analyses of the physicochemical properties of cheese and whey, microbiological quality of cheese, as well as antioxidant activity and total content of polyphenols in cheese and whey were financed by the National Science Centre, Poland–MINIATURA 7, grant no. 2023/07/X/NZ9/00618. The analyses of the polyphenolic profile in nettle leaves, whey, and cheese as well as the sensory quality of cheese were supported by funds from the project: the task titled “Research network of life science universities for the development of the Polish dairy sector—Research project” financed under the targeted subsidy of the Minister of Science and Higher Education (Warsaw, Poland; no. MEiN/2023/DPI/2862). For publication, the funds from the project “Research network of life science universities for the development of the Polish dairy sector—Research project” financed under the targeted subsidy of the Minister of Science and Higher Education (Warsaw, Poland; no. MEiN/2023/DPI/2862) were used. The research activities regarding sensory analysis were approved by the University of Agriculture Ethics Committee (approval no. 189/2024, May 23, 2024, and approval no. 316/2025, September 29, 2025), and all participants provided informed consent. The authors have not stated any conflicts of interest.
utb.wos.affiliation [Pluta-Kubica, A.; Najgebauer-Lejko, D.; Gamage, A. C. K. Aththota; Duda, I.; Domagala, J.] Agr Univ Krakow, Fac Food Technol, Dept Anim Prod Proc, PL-30149 Krakow, Poland; [Pajak, P.; Sobolewska-Zielinska, J.] Agr Univ Krakow, Fac Food Technol, Dept Food Anal & Qual Assessment, PL-30149 Krakow, Poland; [Skoczylas, L.] Agr Univ Krakow, Fac Food Technol, Dept Plant Prod Technol & Nutr Hyg, PL-30149 Krakow, Poland; [Kratochvilova, A.; Salek, R. N.] Tomas Bata Univ Zlin, Fac Technol, Dept Food Technol, Zlin 76001, Czech Republic
utb.fulltext.projects 2023/07/X/NZ9/00618
utb.fulltext.projects MEiN/2023/DPI/2862
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