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Preparation of keratin hydrolysate from chicken feathers and its application in cosmetics

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dc.title Preparation of keratin hydrolysate from chicken feathers and its application in cosmetics en
dc.contributor.author Mokrejš, Pavel
dc.contributor.author Huťťa, Matouš
dc.contributor.author Pavlačková, Jana
dc.contributor.author Egner, Pavlína
dc.relation.ispartof Journal of Visualized Experiments
dc.identifier.issn 1940-087X Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2017
utb.relation.volume 2017
utb.relation.issue 129
dc.type article
dc.language.iso en
dc.publisher Journal of Visualized Experiments
dc.identifier.doi 10.3791/56254
dc.relation.uri https://www.jove.com/video/56254/preparation-keratin-hydrolysate-from-chicken-feathers-its-application
dc.subject Alkaline-enzyme hydrolysis en
dc.subject Biochemistry en
dc.subject Chicken feathers en
dc.subject Cosmetic formulation en
dc.subject Humectant en
dc.subject Hydration en
dc.subject Issue 129 en
dc.subject Keratin en
dc.subject Keratin hydrolysate en
dc.subject Ointment base en
dc.subject Transepidermal water loss en
dc.description.abstract Keratin hydrolysates (KHs) are established standard components in hair cosmetics. Understanding the moisturizing effects of KH is advantageous for skin-care cosmetics. The goals of the protocol are: (1) to process chicken feathers into KH by alkaline-enzymatic hydrolysis and purify it by dialysis, and (2) to test if adding KH into an ointment base (OB) increases hydration of the skin and improves skin barrier function by diminishing transepidermal water loss (TEWL). During alkaline-enzymatic hydrolysis feathers are first incubated at a higher temperature in an alkaline environment and then, under mild conditions, hydrolyzed with proteolytic enzyme. The solution of KH is dialyzed, vacuum dried, and milled to a fine powder. Cosmetic formulations comprising from oil in water emulsion (O/W) containing 2, 4, and 6 weight% of KH (based on the weight of the OB) are prepared. Testing the moisturizing properties of KH is carried out on 10 men and 10 women at time intervals of 1, 2, 3, 4, 24, and 48 h. Tested formulations are spread at degreased volar forearm sites. The skin hydration of stratum corneum (SC) is assessed by measuring capacitance of the skin, which is one of the most world-wide used and simple methods. TEWL is based on measuring the quantity of water transported per a defined area and period of time from the skin. Both methods are fully non-invasive. KH makes for an excellent occlusive; depending on the addition of KH into OB, it brings about a 30% reduction in TEWL after application. KH also functions as a humectant, as it binds water from the lower layers of the epidermis to the SC; at the optimum KH addition in the OB, up to 19% rise in hydration in men and 22% rise in women occurs. © 2017 Journal of Visualized Experiments. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1007683
utb.identifier.obdid 43877045
utb.identifier.scopus 2-s2.0-85037647244
utb.identifier.wok 000417688700036
utb.source j-scopus
dc.date.accessioned 2018-01-15T16:31:39Z
dc.date.available 2018-01-15T16:31:39Z
utb.contributor.internalauthor Mokrejš, Pavel
utb.contributor.internalauthor Huťťa, Matouš
utb.contributor.internalauthor Pavlačková, Jana
utb.contributor.internalauthor Egner, Pavlína
utb.fulltext.affiliation Pavel Mokrejš1, Matouš Huťťa1, Jana Pavlačková2, Pavlína Egner2 1 Department of Polymer Engineering, Faculty of Technology, Tomas Bata University in Zlín 2 Department of Fat, Tenside and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín Correspondence to: Pavel Mokrejš at mokrejs@ft.utb.cz
utb.fulltext.dates Date Published: 11/27/2017
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utb.fulltext.sponsorship This article was written with support of the project IGA/FT/2017/007 of Tomas Bata University in Zlin.
utb.wos.affiliation [Mokrejs, Pavel; Hut't'a, Matous] Tomas Bata Univ Zlin, Fac Technol, Dept Polymer Engn, Zlin, Czech Republic; [Pavlackova, Jana; Egner, Pavlina] Tomas Bata Univ Zlin, Fac Technol, Dept Fat Tenside & Cosmet Technol, Zlin, Czech Republic
utb.scopus.affiliation Department of Polymer Engineering, Faculty of Technology, Tomas Bata University in Zlín, Czech Republic; Department of Fat, Tenside and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín, Czech Republic
utb.fulltext.projects IGA/FT/2017/007
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