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RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector

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dc.title RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector en
dc.contributor.author Jandera, Pavel
dc.contributor.author Škeříková, Veronika
dc.contributor.author Řehová, Lucie
dc.contributor.author Hájek, Tomáš
dc.contributor.author Baldrianová, Lucie
dc.contributor.author Škopová, Gabriela
dc.contributor.author Kellner, Vladimír
dc.contributor.author Horna, Aleš
dc.relation.ispartof Journal of Separation Science
dc.identifier.issn 1615-9306 OCLC, Ulrich, Sherpa/RoMEO, JCR
dc.date.issued 2005-06
utb.relation.volume 28
utb.relation.issue 9-10
dc.citation.spage 1005
dc.citation.epage 1022
dc.type article
dc.language.iso en
dc.publisher Wiley-VCH Verlag GmbH & Co. en
dc.identifier.doi 10.1002/jssc.200500003
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1002/jssc.200500003/abstract
dc.subject phenolic compounds en
dc.subject flavonoids en
dc.subject natural antioxidants en
dc.subject coulometric detection en
dc.subject beer en
dc.subject wine en
dc.subject tea en
dc.subject yacon en
dc.description.abstract Methods were developed for the analysis of natural antioxidants including phenolic compounds and flavonoids in beverages and plant extracts using gradient HPLC with multi-channel electrochemical coulometric detection. Suitability of various reversed-phase columns for this purpose was compared; pH and mobile phase gradients were optimized with respect to the separation selectivity and sensitivity of detection. Because of different target compounds in various sample types, the overlapping resolution maps and the normalized resolution product approaches described earlier were used to select optimum columns and gradients to suit the analysis of the individual sample types. The methods were applied to the analysis of phenolic compounds and flavonoids in beer, wine, tea, and yacon extracts. 32 phenolic compounds were identified and determined, including derivatives of benzoic and cinnamic acids, flavones, and a few related glycosides. Eight-channel CoulArray detection offers high selectivity and sensitivity with limits of detection in the low mu g L-1 range, at least an order of magnitude lower than single-channel coulometric detection using the Coulochem detector. No special sample pretreatment is necessary and, because of the compatibility of the CoulArray detector with gradient elution, phenolic antioxidants of different polarities can be determined in a single run. In addition to the retention times, the ratios of the areas of the pre-dominant and post-dominant peaks to the area of the dominant peak can be used for improved identification of natural antioxidants. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1002059
utb.identifier.scopus 2-s2.0-21444453441
utb.identifier.wok 000230224300022
utb.identifier.pubmed 16013828
utb.identifier.coden JSSCC
utb.source j-wok
dc.date.accessioned 2011-08-16T15:06:19Z
dc.date.available 2011-08-16T15:06:19Z
utb.contributor.internalauthor Horna, Aleš
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