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The determination of cystatin C in biological samples via the surface plasmon resonance method

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dc.title The determination of cystatin C in biological samples via the surface plasmon resonance method en
dc.contributor.author Lesňák, Michal
dc.contributor.author Jursa, Dominik
dc.contributor.author Miškay, Marek
dc.contributor.author Riedlová, Helena
dc.contributor.author Barčová, Karla
dc.contributor.author Adámek, Milan
dc.relation.ispartof BioTechniques
dc.identifier.issn 0736-6205 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2021
utb.relation.volume 70
utb.relation.issue 5
dc.type article
dc.language.iso en
dc.publisher Future Science Ltd
dc.identifier.doi 10.2144/btn-2020-0151
dc.relation.uri https://www.future-science.com/doi/10.2144/btn-2020-0151
dc.subject biochip en
dc.subject cystatin C en
dc.subject plasmon en
dc.subject SPR en
dc.subject urine en
dc.description.abstract Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C. METHOD SUMMARY Surface plasmon resonance is a sensitive optical method that uses the generation of electromagnetic waves (plasmons) for detection. The propagation of surface plasmons at the metal-dielectric interface is very sensitive to changes in the refractive index, which allows for the monitoring of substance properties very close to the interface. The main advantage of the sensor over other currently used techniques is the possibility of label-free analysis and real-time analysis, with high sensitivity and specificity of the optical method. en
utb.faculty Faculty of Applied Informatics
dc.identifier.uri http://hdl.handle.net/10563/1010370
utb.identifier.obdid 43883348
utb.identifier.scopus 2-s2.0-85107084804
utb.identifier.wok 000766777700001
utb.identifier.pubmed 33998838
utb.identifier.coden BTNQD
utb.source j-scopus
dc.date.accessioned 2021-06-22T16:32:29Z
dc.date.available 2021-06-22T16:32:29Z
dc.description.sponsorship Nanotechnology Centre, VSB - Technical University of Ostrava [SGS SP 2020/20]
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.access openAccess
utb.contributor.internalauthor Adámek, Milan
utb.fulltext.sponsorship The authors would like to acknowledge support from the Nanotechnology Centre, VSB – Technical University of Ostrava for project SGS SP 2020/20. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
utb.wos.affiliation [Lesnak, Michal; Jursa, Dominik; Miskay, Marek; Riedlova, Helena; Barcova, Karla] VSB Tech Univ Ostrava, Fac Safety Engn, Lumirova 13, Ostrava 70030, Czech Republic; [Lesnak, Michal] VSB Tech Univ Ostrava, Nanotechnol Ctr, 17 Listopadu 15, Ostrava 70833, Czech Republic; [Adamek, Milan] Tomas Bata Univ Zlin, Fac Appl Informat, Stranemi 4511, Zlin 76005, Czech Republic
utb.scopus.affiliation Faculty of Safety Engineering, VSB – Technical University of Ostrava, Lumirova 13, Ostrava Vyskovice, 700 30, Czech Republic; Nanotechnology Centre, VSB – Technical University of Ostrava, 17, Listopadu 15, Ostrava Poruba, 708 33, Czech Republic; Faculty of Applied Informatics, Tomas Bata University in Zlin, Nad Stranemi 4511, Zlin, 760 05, Czech Republic
utb.fulltext.projects SGS SP 2020/20
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