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Extracellular vesicle isolation yields increased by low-temperature gaseous plasma treatment of polypropylene tubes

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dc.title Extracellular vesicle isolation yields increased by low-temperature gaseous plasma treatment of polypropylene tubes en
dc.contributor.author Resnik, Matic
dc.contributor.author Kovač, Janez
dc.contributor.author Štukelj, Roman
dc.contributor.author Kralj-Iglič, Veronika
dc.contributor.author Humpolíček, Petr
dc.contributor.author Junkar, Ita
dc.relation.ispartof Polymers
dc.identifier.issn 2073-4360 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2020
utb.relation.volume 12
utb.relation.issue 10
dc.citation.spage 1
dc.citation.epage 13
dc.type article
dc.language.iso en
dc.publisher MDPI AG
dc.identifier.doi 10.3390/polym12102363
dc.relation.uri https://www.mdpi.com/2073-4360/12/10/2363
dc.subject atmospheric pressure plasma jets (APPJs) en
dc.subject extracellular vesicles (EVs) en
dc.subject nanostructures en
dc.subject polypropylene (PP) en
dc.description.abstract Novel Extracellular Vesicles (EVs) based diagnostic techniques are promising non-invasive procedures for early stage disease detection which are gaining importance in the medical field. EVs are cell derived particles found in body liquids, especially blood, from which they are isolated for further analysis. However, techniques for their isolation are not fully standardized and require further improvement. Herein modification of polypropylene (PP) tubes by cold Atmospheric Pressure Plasma Jet (APPJ) is suggested to minimize the EVs to surface binding and thus increase EVs isolation yields. The influence of gaseous plasma treatment on surface morphology was studied by Atomic Force Microscopy (AFM), changes in surface wettability by measuring the Water Contact Angle (WCA), while surface chemical changes were analyzed by X-Ray Photoelectron Spectroscopy (XPS). Moreover, PP tubes from different manufacturers were compared. The final isolation yields of EVs were evaluated by flow cytometry. The results of this study suggest that gaseous plasma treatment is an intriguing technique to uniformly alter surface properties of PP tubes and improve EVs isolation yields up to 42%. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1009977
utb.identifier.obdid 43881870
utb.identifier.scopus 2-s2.0-85092797524
utb.identifier.wok 000586167400001
utb.identifier.pubmed 33076317
utb.source j-scopus
dc.date.accessioned 2020-11-05T13:57:25Z
dc.date.available 2020-11-05T13:57:25Z
dc.description.sponsorship Slovenian Research Agency (ARRS)Slovenian Research Agency - Slovenia [PR-06154]
dc.rights Attribution 4.0 International
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Humpolíček, Petr
utb.fulltext.affiliation Matic Resnik 1*, Janez Kovač 1, Roman Štukelj 2, Veronika Kralj-Iglič 2, Petr Humpolíček 3, Ita Junkar 1 1 Department of Surface Engineering, Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia; janez.kovac@ijs.si (J.K.); ita.junkar@ijs.si (I.J.) 2 Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, SI-1000 Ljubljana, Slovenia; roman.stukelj@zf.uni-lj.si (R.Š.); veronika.kralj-iglic@zf.uni-lj.si (V.K.-I.) 3 Centre of Polymer Systems, Polymer Centre, Tomas Bata University in Zlin, T.G. Masaryk Sq. 5555, 760 05 Zlin, Czech Republic; humpolicek@utb.cz * Correspondence: matic.resnik@ijs.si; Tel.: +386-1-477-3885
utb.fulltext.dates Received: 24 September 2020 Accepted: 12 October 2020 Published: 15 October 2020
utb.fulltext.sponsorship This research was funded by the Slovenian Research Agency (ARRS) grant number PR-06154.
utb.wos.affiliation [Resnik, Matic; Kovac, Janez; Junkar, Ita] Jozef Stefan Inst, Dept Surface Engn, Jamova 39, SI-1000 Ljubljana, Slovenia; [Stukelj, Roman; Kralj-Iglic, Veronika] Univ Ljubljana, Fac Hlth Sci, Lab Clin Biophys, Zdravstvena Pot 5, SI-1000 Ljubljana, Slovenia; [Humpolicek, Petr] Tomas Bata Univ Zlin, Polymer Ctr, Ctr Polymer Syst, TG Masaryk Sq 5555, Zlin 76005, Czech Republic
utb.scopus.affiliation Department of Surface Engineering, Jožef Stefan Institute, Jamova 39, Ljubljana, SI-1000, Slovenia; Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, Ljubljana, SI-1000, Slovenia; Centre of Polymer Systems, Polymer Centre, Tomas Bata University in Zlin, T.G. Masaryk Sq. 5555, Zlin, 760 05, Czech Republic
utb.fulltext.projects PR-06154
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