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Enzymatic hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) scaffolds

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dc.title Enzymatic hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) scaffolds en
dc.contributor.author Kovalčík, Adriána
dc.contributor.author Obruča, Stanislav
dc.contributor.author Kalina, Michal
dc.contributor.author Machovský, Michal
dc.contributor.author Enev, Vojtěch
dc.contributor.author Jakešová, Michaela
dc.contributor.author Sobková, Markéta
dc.contributor.author Márová, Ivana
dc.relation.ispartof Materials
dc.identifier.issn 1996-1944 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2020
utb.relation.volume 13
utb.relation.issue 13
dc.citation.spage 1
dc.citation.epage 21
dc.type article
dc.language.iso en
dc.publisher MDPI AG
dc.identifier.doi 10.3390/ma13132992
dc.relation.uri https://www.mdpi.com/1996-1944/13/13/2992
dc.subject degradation kinetics en
dc.subject grape pomace en
dc.subject hydrolysis en
dc.subject mechanical properties en
dc.subject morphology en
dc.subject scaffolds en
dc.subject polyhydroxyalkanoates en
dc.subject thermal properties en
dc.description.abstract Polyhydroxyalkanoates (PHAs) are hydrolyzable bio-polyesters. The possibility of utilizing lignocellulosic waste by-products and grape pomace as carbon sources for PHA biosynthesis was investigated. PHAs were biosynthesized by employing Cupriavidus necator grown on fructose (PHBV-1) or grape sugar extract (PHBV-2). Fifty grams of lyophilized grape sugar extract contained 19.2 g of glucose, 19.1 g of fructose, 2.7 g of pectin, 0.52 g of polyphenols, 0.51 g of flavonoids and 7.97 g of non-identified rest compounds. The grape sugar extract supported the higher production of biomass and modified the composition of PHBV-2. The biosynthesized PHAs served as matrices for the preparation of the scaffolds. The PHBV-2 scaffolds had about 44.2% lower crystallinity compared to the PHBV-1 scaffolds. The degree of crystallinity markedly influenced the mechanical behavior and enzymatic hydrolysis of the PHA scaffolds in the synthetic gastric juice and phosphate buffer saline solution with the lipase for 81 days. The higher proportion of amorphous moieties in PHBV-2 accelerated enzymatic hydrolysis. After 81-days of lasting enzymatic hydrolysis, the morphological changes of the PHBV-1 scaffolds were negligible compared to the visible destruction of the PHBV-2 scaffolds. These results indicated that the presence of pectin and phenolic moieties in PHBV may markedly change the semi-crystalline character of PHBV, as well as its mechanical properties and the course of abiotic or enzymatic hydrolysis. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1009804
utb.identifier.obdid 43881893
utb.identifier.scopus 2-s2.0-85087970960
utb.identifier.wok 000552315700001
utb.identifier.pubmed 32635613
utb.source j-scopus
dc.date.accessioned 2020-07-29T07:30:39Z
dc.date.available 2020-07-29T07:30:39Z
dc.description.sponsorship project SoMoPro [6SA18032]; European Union's Horizon 2020 Research and Innovation Program - South Moravian Region [665860]; project "Centre of Polymer System plus" - Ministry of Education, Youth and Sports of the Czech Republic-Program NPU I [LO1504]
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Machovský, Michal
utb.fulltext.affiliation Adriana Kovalcik 1*, Stanislav Obruca 1, Michal Kalina 2, Michal Machovsky 3, Vojtech Enev 2, Michaela Jakesova 1, Marketa Sobkova 1, Ivana Marova 1 1 Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic; obruca@fch.vut.cz (S.O.); xcjakesova@vutbr.cz (M.J.); xcsobkovama@vutbr.cz (M.S.); marova@fch.vut.cz (I.M.) 2 Department of Physical and Applied Chemistry, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic; kalina-m@fch.vut.cz (M.K.); enev@fch.vut.cz (V.E.) 3 Centre of Polymer Systems, Tomas Bata University in Zlin, trida Tomase Bati 5678, 760 01 Zlin, Czech Republic; machovsky@utb.cz * Correspondence: kovalcik@fch.vut.cz; Tel.: +420-541-149-422
utb.fulltext.dates Received: 11 June 2020 Accepted: 3 July 2020 Published: 5 July 2020
utb.fulltext.sponsorship This study was funded through the project SoMoPro (project No. 6SA18032). This project has received funding fromthe European Union's Horizon 2020 Research and Innovation Program under the Marie Sk?odowska-Curie, and it is co-financed by the South Moravian Region under grant agreement No. 665860. Michal Machovsky, appreciates the support from the project "Centre of Polymer System plus" funded by the Ministry of Education, Youth and Sports of the Czech Republic-Program NPU I (project number: LO1504). Note: Authors confirm that the content of this work reflects only the author's view and that the E.U. is not responsible for any use that may be made of the information it contains. The authors express their Acknowledgements to Iva Pernicova for assistance with gas chromatography.
utb.wos.affiliation [Kovalcik, Adriana; Obruca, Stanislav; Jakesova, Michaela; Sobkova, Marketa; Marova, Ivana] Brno Univ Technol, Fac Chem, Dept Food Chem & Biotechnol, Purkynova 118, Brno 61200, Czech Republic; [Kalina, Michal; Enev, Vojtech] Brno Univ Technol, Fac Chem, Dept Phys & Appl Chem, Purkynova 118, Brno 61200, Czech Republic; [Machovsky, Michal] Tomas Bata Univ Zlin, Ctr Polymer Syst, Trida Tomase Bati 5678, Zlin 76001, Czech Republic
utb.scopus.affiliation Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, Brno, 612 00, Czech Republic; Department of Physical and Applied Chemistry, Faculty of Chemistry, Brno University of Technology, Purkynova 118, Brno, 612 00, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlin, trida Tomase Bati 5678, Zlin, 760 01, Czech Republic
utb.fulltext.projects 6SA18032
utb.fulltext.projects 665860
utb.fulltext.projects LO1504
utb.fulltext.ou Centre of Polymer Systems
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