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Bovine decellularized amniotic membrane: Extracellular matrix as scaffold for mammalian skin

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dc.title Bovine decellularized amniotic membrane: Extracellular matrix as scaffold for mammalian skin en
dc.contributor.author Villamil Ballesteros, Andrea Catalina
dc.contributor.author Segura Puello, Hugo Ramiro
dc.contributor.author López García, Jorge Andrés
dc.contributor.author Bernal-Ballen, Andres
dc.contributor.author Nieto Mosquera, Diana Lorena
dc.contributor.author Munoz Forero, Diana Milena
dc.contributor.author Segura Charry, Juan Sebastian
dc.contributor.author Neira Bejarano, Yuli Alexandra
dc.relation.ispartof Polymers
dc.identifier.issn 2073-4360 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2020
utb.relation.volume 12
utb.relation.issue 3
dc.type article
dc.language.iso en
dc.publisher Mdpi
dc.identifier.doi 10.3390/polym12030590
dc.relation.uri https://www.mdpi.com/2073-4360/12/3/590
dc.subject decellularization en
dc.subject biological scaffolding en
dc.subject bovine amniotic membrane en
dc.subject extracellular matrix en
dc.subject tissue regeneration en
dc.description.abstract Decellularized membranes (DM) were obtained from bovine amniotic membranes (BAM) using four different decellularization protocols, based on physical, chemical, and mechanical treatment. The new material was used as a biological scaffold for in vitro skin cell culture. The DM were characterized using hematoxylin-eosin assay, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR-ATR), and differential scanning calorimetry (DSC). The in vitro cytotoxicity of DM was evaluated using MTT. The efficacy of decellularization process was assessed throughDNAquantification and electrophoresis. All the used protocols showed a high effectiveness in terms of elimination of native cells, confirmed by DNA extraction and quantification, electrophoresis, and SEM, although protocol IV removes the cellular contents and preserve the native extracellular matrix (ECM) architecture which it can be considered as the most effective in terms of decellularization. FTIR-ATR and DSC on the other hand, revealed the effects of decellularization on the biochemical composition of the matrices. There was no cytotoxicity and the biological matrices obtained were a source of collagen for recellularization. The matrices of protocols I, II, and III were degraded at day 21 of cell culture, forming a gel. The biocompatibility in vitro was demonstrated; hence these matrices may be deemed as potential scaffold for epithelial tissue regeneration. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1009648
utb.identifier.obdid 43881909
utb.identifier.scopus 2-s2.0-85082671279
utb.identifier.wok 000525952000089
utb.identifier.pubmed 32151022
utb.source J-wok
dc.date.accessioned 2020-05-06T19:41:52Z
dc.date.available 2020-05-06T19:41:52Z
dc.rights Attribution 4.0 International
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor López García, Jorge Andrés
utb.fulltext.affiliation Andrea Catalina Villamil Ballesteros 1*, Hugo Ramiro Segura Puello 1, Jorge Andres Lopez-Garcia 2, Andres Bernal-Ballen 3, Diana Lorena Nieto Mosquera 1, Diana Milena Muñoz Forero 1, Juan Sebastián Segura Charry 1, Yuli Alexandra Neira Bejarano 1 1 Laboratorio de Investigaciones en Salud, Universidad Manuela Beltrán, Avenida Circunvalar No. 60-00, Bogotá 110231, Colombia; hugo.segura@umb.edu.co (H.R.S.P.); orena.nieto@umb.edu.co (D.L.N.M.); diana.munoz@umb.edu.co (D.M.M.F.); reumatologiasegura@hotmail.com (J.S.S.C.); yuli.neira@umb.edu.co (Y.A.N.B.) 2 Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 76001 Zlín, Czech Republic; vextropk@gmail.com 3 Grupo de Investigación en Ingeniería Biomédica, Vicerrectoría de Investigaciones, Universidad Manuela Beltrán, Avenida Circunvalar No. 60-00, Bogotá 110231, Colombia; andres.bernal@docentes.umb.edu.co * Correspondence: gemecata15@hotmail.com
utb.fulltext.dates Received: 31 August 2019 Accepted: 23 November 2019 Published: 5 March 2020
utb.wos.affiliation [Villamil Ballesteros, Andrea Catalina; Segura Puello, Hugo Ramiro; Nieto Mosquera, Diana Lorena; Munoz Forero, Diana Milena; Segura Charry, Juan Sebastian; Neira Bejarano, Yuli Alexandra] Univ Manuela Beltran, Lab Invest Salud, Ave Circunvalar 60-00, Bogota 110231, Colombia; [Lopez-Garcia, Jorge Andres] Tomas Bata Univ Zlin, Univ Inst, Ctr Polymer Syst, Trida Tomase Bati 5678, Zlin 76001, Czech Republic; [Bernal-Ballen, Andres] Univ Manuela Beltran, Vicerrectoria Invest, Grp Invest Ingn Biomed, Ave Circunvalar 60-00, Bogota 110231, Colombia
utb.scopus.affiliation Laboratorio de Investigaciones en Salud, Universidad Manuela Beltrán, Avenida Circunvalar No. 60-00, Bogotá, 110231, Colombia; Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, 76001, Czech Republic; Grupo de Investigación en Ingeniería Biomédica, Vicerrectoría de Investigaciones, Universidad Manuela Beltrán, Avenida Circunvalar No. 60-00, Bogotá, 110231, Colombia
utb.fulltext.faculty University Institute
utb.fulltext.ou Centre of Polymer Systems
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