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Tunable biomimetic hydrogels from silk fibroin and nanocellulose

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dc.title Tunable biomimetic hydrogels from silk fibroin and nanocellulose en
dc.contributor.author Dorishetty, Pramod
dc.contributor.author Balu, Rajkamal
dc.contributor.author Athukoralalage, Sandya S.
dc.contributor.author Greaves, Tamar L.
dc.contributor.author Mata, Jitendra
dc.contributor.author De Campo, Liliana
dc.contributor.author Saha, Nabanita
dc.contributor.author Zannettino, Andrew C.W.
dc.contributor.author Dutta, Naba K.
dc.contributor.author Choudhury, Namita Roy
dc.relation.ispartof ACS Sustainable Chemistry and Engineering
dc.identifier.issn 2168-0485 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2020
utb.relation.volume 8
utb.relation.issue 6
dc.citation.spage 2375
dc.citation.epage 2389
dc.type article
dc.language.iso en
dc.publisher American Chemical Society
dc.identifier.doi 10.1021/acssuschemeng.9b05317
dc.relation.uri https://pubs.acs.org/doi/10.1021/acssuschemeng.9b05317
dc.subject silk fibroin en
dc.subject nanocellulose en
dc.subject composite hydrogel en
dc.subject hierarchical structure en
dc.subject mechanical properties en
dc.subject cellular response en
dc.subject 3D printing en
dc.description.abstract Biomimetic hydrogels offer a new platform for hierarchical structure-controlled, tough, biocompatible, mechanically tunable, and printable gels for regenerative medicine. Herein, we report for the first time the detailed effects of various kinds of nanocellulose, namely, bacterial nanocellulose, cellulose nanofibers, and cellulose nanocrystals on the morphology, structure-property relationship, and 3D printability of the photochemically cross-linked regenerated silk fibroin (RSF)/nanocellulose composite hydrogels. The hierarchical structure of fabricated biomimetic hydrogels was both qualitatively and quantitatively investigated by scanning electron microscopy and small/ultrasmall-angle neutron scattering, whereas their mechanical properties were assessed using rheology, tensile, and indentation tests. The micropore size and interhydrophobic domain distance of fabricated hydrogels were tuned in the range of 1.8-9.2 μm and 4.5-17.7 nm, respectively. The composite hydrogels exhibit superior viscoelastic, compressive, and tensile mechanical properties compared to pristine RSF hydrogel, where the shear storage modulus, compression modulus, young's modulus, and tensile toughness were tuned in the range of 0.4-1.4, 1.3-3.6, 2.2-14.0 MPa, and 16.7-108.3 kJ/m3, respectively. Moreover, the obtained mechanical modulus of the composite hydrogels in terms of shear, tensile, and compression are comparable to articular cartilage (0.4-1.6 MPa), native femoral artery (∼9.0 MPa), and human medial meniscus (∼1.0 MPa) tissues, respectively, which demonstrate their potential for a wide range of tissue engineering applications. The whisker form of nanocellulose was observed to enhance the printability of composite hydrogels, whereas the fiber form enhanced the overall toughness of the composite hydrogels and promoted the fibroblast cell attachment, viability, and proliferation. The results presented here have implications for both fundamental understanding and potential applications of RSF/nanocellulose composite hydrogels for 3D-printed scaffolds and tissue engineering. Copyright © 2020 American Chemical Society. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1009602
utb.identifier.obdid 43881899
utb.identifier.scopus 2-s2.0-85080066720
utb.identifier.wok 000514488600005
utb.source j-scopus
dc.date.accessioned 2020-03-12T14:04:40Z
dc.date.available 2020-03-12T14:04:40Z
dc.description.sponsorship Australian Research Council (ARC)Australian Research Council [DP160101267]; ANSTO beam time award [P5772]; NSFNational Science Foundation (NSF) [DMR-0520547]; European UnionEuropean Union (EU) [654000]
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Saha, Nabanita
utb.fulltext.affiliation Pramod Dorishetty, Rajkamal Balu, Sandya S. Athukoralalage, Tamar L. Greaves, Jitendra Mata, Liliana de Campo, Nabanita Saha, Andrew C. W. Zannettino, Naba K. Dutta *, Namita Roy Choudhury * Pramod Dorishetty − School of Engineering, RMIT University, Melbourne, Victoria 3000, Australia; orcid.org/0000-0002-3378-6172 Rajkamal Balu − School of Engineering, RMIT University, Melbourne, Victoria 3000, Australia Sandya S. Athukoralalage − School of Engineering, RMIT University, Melbourne, Victoria 3000, Australia Tamar L. Greaves − School of Science, RMIT University, Melbourne, Victoria 3000, Australia; orcid.org/0000-0002-0298-0183 Jitendra Mata − Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights, New South Wales 2234, Australia; orcid.org/0000-0001-9225-7900 Liliana de Campo − Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights, New South Wales 2234, Australia Nabanita Saha − Centre of Polymer Systems, Tomas Bata University in Zlin, Zlin 76001, Czech Republic Andrew C. W. Zannettino − Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia; Central Adelaide Local Health Network, Adelaide, South Australia 5000, Australia * Corresponding Authors Naba K. Dutta − School of Engineering, RMIT University, Melbourne, Victoria 3000, Australia; School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, South Australia 5005, Australia; orcid.org/0000-0003-4800-1910; Email: naba.dutta@rmit.edu.au Namita Roy Choudhury − School of Engineering, RMIT University, Melbourne, Victoria 3000, Australia; School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, South Australia 5005, Australia;orcid.org/0000-0001-9010-8506; Email: namita.choudhury@rmit.edu.au
utb.fulltext.dates Received: September 7, 2019 Revised: January 18, 2020 Published: January 23, 2020
utb.fulltext.sponsorship This research has been financially supported by the Australian Research Council (ARC) through the Discovery research grant (DP160101267). Access to the SANS and USANS facility at the Australian Centre for Neutron Scattering was supported through the ANSTO beam time award (P5772). The authors acknowledge the facilities, as well as scientific and technical assistance of the Adelaide Microscopy (The University of Adelaide, Adelaide), BioFab3D (St. Vincent’s Hospital, Melbourne), Micro−Nano Research Facility, and Centre for Nanoscale Biophotonics and Microscopy and Microanalysis Facility (RMIT University, Melbourne). This work benefited from the use of SasView application, originally developed under NSF award DMR-0520547. SasView contains the code developed with funding from the European Union’s Horizon 2020 research and innovation programme under the SINE2020 project, grant agreement no. 654000.
utb.wos.affiliation [Dorishetty, Pramod; Balu, Rajkamal; Athukoralalage, Sandya S.; Dutta, Naba K.; Choudhury, Namita Roy] RMIT Univ, Sch Engn, Melbourne, Vic 3000, Australia; [Dutta, Naba K.; Choudhury, Namita Roy] Univ Adelaide, Sch Chem Engn & Adv Mat, Adelaide, SA 5005, Australia; [Greaves, Tamar L.] RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia; [Mata, Jitendra; de Campo, Liliana] ANSTO, Australian Ctr Neutron Scattering, Lucas Heights, NSW 2234, Australia; [Saha, Nabanita] Tomas Bata Univ Zlin, Ctr Polymer Syst, Zlin 76001, Czech Republic; [Zannettino, Andrew C. W.] Univ Adelaide, Fac Hlth & Med Sci, Adelaide, SA 5005, Australia; [Zannettino, Andrew C. W.] Cent Adelaide Local Hlth Network, Adelaide, SA 5000, Australia
utb.scopus.affiliation School of Engineering, RMIT University, La Trobe Street, Melbourne, VIC 3000, Australia; School of Science, RMIT University, La Trobe Street, Melbourne, VIC 3000, Australia; Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation (ANSTO), New Illawarra Road, Lucas Heights, NSW 2234, Australia; Centre of Polymer Systems, Tomas Bata University in Zlin, Nam. T. G. Masaryka 5555, Zlin, 76001, Czech Republic; Faculty of Health and Medical Sciences, The University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; School of Chemical Engineering and Advanced Materials, The University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; Central Adelaide Local Health Network, Adelaide, SA 5000, Australia
utb.fulltext.projects DP160101267
utb.fulltext.projects P5772
utb.fulltext.projects DMR-0520547
utb.fulltext.projects SINE2020
utb.fulltext.projects 654000
utb.fulltext.ou Centre of Polymer Systems
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