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Biocompatibility and biological efficiency of inorganic calcium filled bacterial cellulose based hydrogel scaffolds for bone bioengineering

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dc.title Biocompatibility and biological efficiency of inorganic calcium filled bacterial cellulose based hydrogel scaffolds for bone bioengineering en
dc.contributor.author Basu, Probal
dc.contributor.author Saha, Nabanita
dc.contributor.author Alexandrova, Radostina I.
dc.contributor.author Andonova-Lilova, Boyka
dc.contributor.author Georgieva, Milena G.
dc.contributor.author Miloshev, George
dc.contributor.author Sáha, Petr
dc.relation.ispartof International Journal of Molecular Sciences
dc.identifier.issn 1661-6596 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2018
utb.relation.volume 19
utb.relation.issue 12
dc.type article
dc.language.iso en
dc.publisher Molecular Diversity Preservation International (MDPI)
dc.identifier.doi 10.3390/ijms19123980
dc.relation.uri https://www.mdpi.com/1422-0067/19/12/3980
dc.subject Apoptosis en
dc.subject Bacterial cellulose en
dc.subject Biocompatibility en
dc.subject Bone tissue engineering en
dc.subject DNA damage en
dc.subject In vitro bio-mineralization en
dc.description.abstract The principal focus of this work is the in-depth analysis of the biological efficiency of inorganic calcium-filled bacterial cellulose (BC) based hydrogel scaffolds for their future use in bone tissue engineering/bioengineering. Inorganic calcium was filled in the form of calcium phosphate (β-tri calcium phosphate (β-TCP) and hydroxyapatite (HA)) and calcium carbonate (CaCO3). The additional calcium, CaCO3 was incorporated following in vitro bio-mineralization. Cell viability study was performed with the extracts of BC based hydrogel scaffolds: BC-PVP, BC-CMC; BC-PVP-β-TCP/HA, BC-CMC-β-TCP/HA and BC-PVP-β-TCP/HA-CaCO3, BC-CMC-β-TCP/HA-CaCO3; respectively. The biocompatibility study was performed with two different cell lines, i.e., human fibroblasts, Lep-3 and mouse bone explant cells. Each hydrogel scaffold has facilitated notable growth and proliferation in presence of these two cell types. Nevertheless, the percentage of DNA strand breaks was higher when cells were treated with BC-CMC based scaffolds i.e., BC-CMC-β-TCP/HA and BC-CMC-β-TCP/HA-CaCO3. On the other hand, the apoptosis of human fibroblasts, Lep-3 was insignificant in BC-PVP-β-TCP/HA. The scanning electron microscopy confirmed the efficient adhesion and growth of Lep-3 cells throughout the surface of BC-PVP and BC-PVP-β-TCP/HA. Hence, among all inorganic calcium filled hydrogel scaffolds, ‘BC-PVP-β-TCP/HA’ was recommended as an efficient tissue engineering scaffold which could facilitate the musculoskeletal (i.e., bone tissue) engineering/bioengineering. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1008366
utb.identifier.obdid 43879346
utb.identifier.scopus 2-s2.0-85058421915
utb.identifier.wok 000455323500282
utb.identifier.pubmed 30544895
utb.source j-scopus
dc.date.accessioned 2019-01-31T08:58:57Z
dc.date.available 2019-01-31T08:58:57Z
dc.description.sponsorship Internal Grant Agency, Tomas Bata University in Zlin, Czech Republic [IGA/CPS/2017/003, IGA/CPS/2018/008]; Ministry of Education, Youth and Sports of the Czech Republic-NPU Program I [LO1504]; National Scientific Fund, Bulgarian Ministry of Education and Science [DFNI (sic) 02 30]; Bulgarian Science Fund [DN 11/15]; NATO science Peace and Security program [NATO SPS MYP G5266]; COST Action [CA15214, MP1301]
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Basu, Probal
utb.contributor.internalauthor Saha, Nabanita
utb.contributor.internalauthor Sáha, Petr
utb.scopus.affiliation Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Trida Tomase Bati 5678, Zlín, 760 01, Czech Republic; Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria; Laboratory of Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International