Green synthesis of silver nanoparticles and biopolymer nanocomposites: a comparative study on physico-chemical, antimicrobial and anticancer activity

Palem, Ramasubba Reddy; Shimoga Dinesh, Ganesh; Kroneková, Zuzana; Sláviková, Monika; Saha, Nabanita; Sáha, Petr

dc.title	Green synthesis of silver nanoparticles and biopolymer nanocomposites: a comparative study on physico-chemical, antimicrobial and anticancer activity	en
dc.contributor.author	Palem, Ramasubba Reddy
dc.contributor.author	Shimoga Dinesh, Ganesh
dc.contributor.author	Kroneková, Zuzana
dc.contributor.author	Sláviková, Monika
dc.contributor.author	Saha, Nabanita
dc.contributor.author	Sáha, Petr
dc.relation.ispartof	Bulletin of Materials Science
dc.identifier.issn	0250-4707 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2018
utb.relation.volume	41
utb.relation.issue	2
dc.type	article
dc.language.iso	en
dc.publisher	Indian Academy of Sciences
dc.identifier.doi	10.1007/s12034-018-1567-5
dc.relation.uri	https://link.springer.com/article/10.1007/s12034-018-1567-5
dc.subject	Biogenic silver nanoparticles	en
dc.subject	biopolymer nanocomposites	en
dc.subject	nanoparticles stability	en
dc.subject	antimicrobial activity	en
dc.subject	anticancer activity	en
dc.description.abstract	The current report was intended towards comparative study of green-synthesized biogenic Rhubarb silver nanoparticles (RS-AgNPs) and chitosan crosslinked silver nanocomposites (CSHD-AgNCs). The physico-chemical characterization was done by UV-visible, FTIR, scanning electron microscopy (SUM), transmission electron microscopy (TEM), TGA, XRD and zeta potential(zeta). The analysis and spectroscopic characterization was done by SEM and TEM and their results reveal that the nanoparticles are spherical in shape, with average size ranges from 5 to 50 nm, and was gathered by face centered cubic (FCC) structure throughout the polymer matrix and stable without any protecting or capping reagents over 450 days. The antimicrobial property of RS-AgNPs and CSHD-AgNCs (zeta = +29.6 and +32.8 mV) was evaluated against E. coli and S. aureus and showed an effective inhibitory property. The RS-AgNPs and CSHD-AgNCs were assessed for their anticancer activity against HeLa cell line by MTT method, and it reveals a dose response activity, time and cell line-dependent cytotoxicity. Based on the results obtained, the RS-AgNPs exhibited higher toxicity over CSHD-AgNCs after 24 h incubation of HeLa cells with different concentrations and is negligible for the aqueous Rhubarb extract. It was concluded that the changes in anticancer activity towards HeLa cells due to biological activity of silver nanoparticles depend on their method of biosynthesis and their physico-chemical nature.	en
utb.faculty	University Institute
dc.identifier.uri	http://hdl.handle.net/10563/1007928
utb.identifier.obdid	43878242
utb.identifier.scopus	2-s2.0-85048642337
utb.identifier.wok	000430795900020
utb.source	j-wok
dc.date.accessioned	2018-05-18T15:12:09Z
dc.date.available	2018-05-18T15:12:09Z
dc.description.sponsorship	Ministry of Education, Youth and Sports of the Czech Republic-NPU Program I [LO1504]; Slovak Grant Agency VEGA [2/0124/18]
utb.ou	Centre of Polymer Systems
utb.contributor.internalauthor	Palem, Ramasubba Reddy
utb.contributor.internalauthor	Shimoga Dinesh, Ganesh
utb.contributor.internalauthor	Saha, Nabanita
utb.contributor.internalauthor	Sáha, Petr
utb.fulltext.affiliation	RAMASUBBA REDDY PALEM1, SHIMOGA D GANESH1, ZUZANA KRONEKOVA2, MONIKA SLÁVIKOVÁ3, NABANITA SAHA1,∗ and PETR SAHA1 1 Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, 760 01 Zlin, Czech Republic 2 Department for Biomaterials Research, Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, 845 41 Bratislava, Slovakia 3 Institute of Virology, Biomedical Centre, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovakia ∗ Author for correspondence (nabanita@utb.cz)
utb.fulltext.dates	MS received 3 March 2017 accepted 12 August 2017 published online 29 March 2018
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utb.fulltext.sponsorship	We are grateful to the Ministry of Education, Youth and Sports of the Czech Republic—NPU Program I (LO1504) and Slovak Grant Agency VEGA for the financial support in the Project 2/0124/18. We are also thankful to Department of Material Science and Nanotechnology (IISc) Bangalore, India, for TEM analysis.
utb.wos.affiliation	[Palem, Ramasubba Reidy; Ganesh, Shimoga D.; Saha, Nabanita; Saha, Petr] Tomas Bata Univ Zlin, Univ Inst, Ctr Polymer Syst, Tr T Bati 5678, Zlin 76001, Czech Republic; [Kronekova, Zuzana] Slovak Acad Sci, Polymer Inst, Dept Biomat Res, Dubrayska Cesta 9, Bratislava 84541, Slovakia; [Slavikova, Monika] Slovak Acad Sci, Biomed Ctr, Inst Virol, Dubrayska Cesta 9, Bratislava 84505, Slovakia
utb.scopus.affiliation	Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, Zlin, 760 01, Czech Republic; Department for Biomaterials Research, Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava, 845 41, Slovakia; Institute of Virology, Biomedical Centre, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava, 845 05, Slovakia
utb.fulltext.projects	LO1504
utb.fulltext.projects	VEGA 2/0124/18