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Ex Vivo and In Vitro Studies on the Cytotoxicity and Immunomodulative Properties of Poly(2-isopropenyl-2-oxazoline) as a New Type of Biomedical Polymer

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dc.title Ex Vivo and In Vitro Studies on the Cytotoxicity and Immunomodulative Properties of Poly(2-isopropenyl-2-oxazoline) as a New Type of Biomedical Polymer en
dc.contributor.author Kroneková, Zuzana
dc.contributor.author Mikulec, Marcel
dc.contributor.author Petrenčíková, Nadežda
dc.contributor.author Paulovičová, Ema
dc.contributor.author Paulovičová, Lucia
dc.contributor.author Jančinová, Viera
dc.contributor.author Nosáľ', Radomír
dc.contributor.author Palem Reddy, Ramasubba
dc.contributor.author Dinesh Shimoga, Ganesh
dc.contributor.author Chorvát, Dušan Jr.
dc.contributor.author Kronek, Juraj
dc.relation.ispartof Macromolecular Bioscience
dc.identifier.issn 1616-5187 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2016
dc.citation.spage 1200
dc.citation.epage 1211
dc.type article
dc.language.iso en
dc.publisher Wiley-VCH Verlag
dc.identifier.doi 10.1002/mabi.201600016
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1002/mabi.201600016/abstract
dc.subject biocompatibility en
dc.subject cell proliferation en
dc.subject fluorescence en
dc.subject functionalization of polymers en
dc.subject poly(2-isopropenyl-2-oxazoline) en
dc.subject splenocytes en
dc.description.abstract Poly(2-alkenyl-2-oxazoline)s are promising functional polymers for a variety of biomedical applications, such as drug delivery systems, peptide conjugates, or gene delivery. In this study, poly(2-isopropenyl-2-oxazoline) (PIPOx) is prepared through free-radical polymerization initiated with azobisisobutyronitrile. Reactive 2-oxazoline units in the side chain support an addition reaction with different compounds containing a carboxylic group, which facilitates the preparation of polymers labeled with two different fluorescent dyes. The cytotoxicities of 2-oxazoline monomers, PIPOx, and fluorescently labeled PIPOx are evaluated in vitro using an 3-(4,5-Dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and ex vivo using a cell proliferation assay with adenosine triphosphate bioluminescence. The cell uptake of labeled PIPOx is used to determine the colocalization of PIPOx with cell organelles that are part of the endocytic pathway. For the first time, it is shown that poly(2-isopropenyl-2-oxazoline) is a biocompatible material and is suitable for biomedical applications; further, its immunomodulative properties are evaluated. (Figure presented.). © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1006636
utb.identifier.obdid 43875272
utb.identifier.scopus 2-s2.0-84982908292
utb.identifier.wok 000381047500009
utb.identifier.pubmed 27150385
utb.identifier.coden MBAIB
utb.source j-scopus
dc.date.accessioned 2016-10-25T12:38:01Z
dc.date.available 2016-10-25T12:38:01Z
dc.description.sponsorship 2/0156/15, VEGA, Vedecká Grantová Agentúra MŠVVaŠ SR a SAV; 2/0163/15, VEGA, Vedecká Grantová Agentúra MŠVVaŠ SR a SAV; APVV-0242-11, APVV, Agentúra na Podporu Výskumu a Vývoja
dc.description.sponsorship Slovak Grant Agency VEGA [2/0163/15, 2/0156/15]; Slovak Research and Development Agency (SRDA) [APVV-0242-11]; Slovak Research and Development Agency [APVV-SK-CZ-2013-0206]; Ministry of Education, Youth and Sports, Czech Republic [MSMT-7AMB14SK026]
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Palem Reddy, Ramasubba
utb.contributor.internalauthor Dinesh Shimoga, Ganesh
utb.fulltext.affiliation Zuzana Kroneková , Marcel Mikulec , Nadežda Petrenčíková , Ema Paulovičová , Lucia Paulovičová , Viera Jančinová , Radomír Nosáľ , Palem S. Reddy , Ganesh D. Shimoga , Dušan Chorvát Jr. , Juraj Kronek * Dr. Z. Kroneková, Dr. M. Mikulec, N. Petrenčíková, Dr. J. Kronek Department for Biomaterials Research Polymer Institute Slovak Academy of Sciences Dubravska cesta 9, 845 41 Bratislava , Slovakia E-mail: juraj.kronek@savba.sk Dr. E. Paulovičová, Dr. L. Paulovičová Institute of Chemistry Center of Glycomics Slovak Academy of Sciences Dubravska cesta 9, 84236 Bratislava , Slovakia Dr. V. Jančinová, Prof. R. Nosáľ Institute of Experimental Pharmacology & Toxicology Slovak Academy of Sciences Dubravska cesta 9, 841 04 Bratislava , Slovakia Dr. P. S. Reddy, Dr. G. D. Shimoga Centre of Polymer Systems University Institute Tomas Bata University in Zlin Třída T. Bati 5678 , Zlin 760 01 , Czech Republic Dr. D. Chorvát Jr. International Laser Centre Ilkovičova 3 , 841 04 Bratislava , Slovakia
utb.fulltext.dates Received: January 12, 2016 ; Revised: April 1, 2016 ; Published online: May 6, 2016
utb.fulltext.sponsorship The authors thank the Slovak Grant Agency VEGA for fi nancial support through the project Nos. 2/0163/15 and 2/0156/15 and the Slovak Research and Development Agency (SRDA) for fi nancial support through the project No. APVV-0242-11 . This work was performed through Slovak-Czech bilateral cooperation under contract No. APVV-SK-CZ-2013-0206 (Slovak Research and Development Agency) and contract No. MSMT-7AMB14SK026 (Ministry of Education, Youth and Sports, Czech Republic).
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