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Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

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dc.title Improved stability and efficacy of chitosan/pDNA complexes for gene delivery en
dc.contributor.author Cifani, Noemi
dc.contributor.author Chronopoulou, Laura
dc.contributor.author Pompili, Barbara
dc.contributor.author Di Martino, Antonio
dc.contributor.author Bordi, Federico
dc.contributor.author Sennato, Simona
dc.contributor.author Di Domenico, Enea Gino
dc.contributor.author Palocci, Cleofe
dc.contributor.author Ascenzioni, Fiorentina
dc.relation.ispartof Biotechnology Letters
dc.identifier.issn 0141-5492 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2015
utb.relation.volume 37
utb.relation.issue 3
dc.citation.spage 557
dc.citation.epage 565
dc.type article
dc.language.iso en
dc.publisher Springer Netherlands
dc.identifier.doi 10.1007/s10529-014-1727-7
dc.relation.uri https://link.springer.com/article/10.1007/s10529-014-1727-7
dc.subject Chitosan en
dc.subject Chitosan/pDNA nanocomplexes en
dc.subject DNA transfection en
dc.subject Gene delivery en
dc.subject HEK293 cells en
dc.subject Transfection efficiency en
dc.subject Therapeutic genes en
dc.description.abstract Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 A degrees C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1004292
utb.identifier.rivid RIV/70883521:28610/15:43872977!RIV16-MSM-28610___
utb.identifier.obdid 43873397
utb.identifier.scopus 2-s2.0-84925491258
utb.identifier.wok 000350394800010
utb.identifier.coden BILED
utb.source j-wok
dc.date.accessioned 2015-05-22T08:01:46Z
dc.date.available 2015-05-22T08:01:46Z
dc.description.sponsorship Istituto Pasteur Fondazione Cenci Bolognetti; Istituto Italiano di Tecnologia (IIT) Center of Life NanoScience-Sapienza
utb.contributor.internalauthor Di Martino, Antonio
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