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In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

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dc.title In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications en
dc.contributor.author Shah, Rushita
dc.contributor.author Kroneková, Zuzana
dc.contributor.author Zahoranová, Anna
dc.contributor.author Roller, Ladislav
dc.contributor.author Saha, Nabanita
dc.contributor.author Sáha, Petr
dc.contributor.author Kronek, Juraj
dc.relation.ispartof Journal of Materials Science : Materials in Medicine
dc.identifier.issn 0957-4530 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2015
utb.relation.volume 26
utb.relation.issue 4
dc.citation.spage 157
dc.type article
dc.language.iso en
dc.publisher Springer
dc.identifier.doi 10.1007/s10856-015-5485-4
dc.relation.uri https://link.springer.com/article/10.1007/s10856-015-5485-4
dc.description.abstract Polymers based on 2-oxazoline, such as poly(2-ethyl-2-oxazolines) (PETOx), are considered to be a type of 'pseudopeptide' with the ability to form novel biomaterials. The hydrolysis of PETOx was carried out to evaluate its use in biomedical applications. In the present work, PETOx samples with a range of molar masses were prepared by living cationic polymerization. Hydrolysis was carried out at time intervals ranging from 15 to 180 min to prepare copolymers with different amounts of ethylene imine units. H-1 NMR spectroscopy was used to identify the structure of the hydrolyzed polymers. The dependence of in vitro cell viability on the degree of hydrolysis was determined using three different model cell lines, namely, mouse embryonic 3T3 fibroblasts, pancreatic beta TC3 cells, and mouse lymphoid macrophages P388.D1. It was demonstrated that increasing the degree of hydrolysis decreased cell viability for all cell types. Fibroblast cells displayed the highest tolerance; additionally, the effect of polymer size showed no observable significance. Macrophage cells, immune system representatives, displayed the highest sensitivity to contact with hydrolyzed PETOx. The effect of polymer hydrolysis, polymer concentration and the incubation time on cell viability was experimentally observed. Confocal laser-scanning microscopy provided evidence of cellular uptake of pyrene-labeled (co)polymers. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1004288
utb.identifier.obdid 43873388
utb.identifier.scopus 2-s2.0-84938089440
utb.identifier.wok 000351584300004
utb.identifier.pubmed 25783502
utb.identifier.coden JSMME
utb.source j-wok
dc.date.accessioned 2015-05-22T08:01:45Z
dc.date.available 2015-05-22T08:01:45Z
dc.description.sponsorship Slovak Grant Agency, VEGA [2/0151/12, 2/0163/12]; Operational Program Education for Competitiveness; European Social Fund (ESF); national budget of the Czech Republic [CZ.1.07/2.3.00/20.0104]; Ministry of Education, Youth and Sports of the Czech Republic [ME-LH14050]
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Shah, Rushita
utb.contributor.internalauthor Saha, Nabanita
utb.contributor.internalauthor Sáha, Petr
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