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Determination of lipidomic differences between human breast cancer and surrounding normal tissues using HILIC-HPLC/ESI-MS and multivariate data analysis

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dc.title Determination of lipidomic differences between human breast cancer and surrounding normal tissues using HILIC-HPLC/ESI-MS and multivariate data analysis en
dc.contributor.author Cífková, Eva
dc.contributor.author Holčapek, Michal
dc.contributor.author Lísa, Miroslav
dc.contributor.author Vrána, David
dc.contributor.author Gatěk, Jiří
dc.contributor.author Melichar, Bohuslav
dc.relation.ispartof Analytical and Bioanalytical Chemistry
dc.identifier.issn 1618-2642 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2015
utb.relation.volume 407
utb.relation.issue 3
dc.citation.spage 991
dc.citation.epage 1002
dc.type article
dc.language.iso en
dc.publisher Springer Heidelberg
dc.identifier.doi 10.1007/s00216-014-8272-z
dc.relation.uri https://link.springer.com/article/10.1007/s00216-014-8272-z
dc.subject Breast cancer en
dc.subject Lipid biomarkers en
dc.subject HILIC-HPLC/ESI-MS en
dc.subject Quantitation en
dc.subject Statistical analysis en
dc.description.abstract The comprehensive approach for the lipidomic characterization of human breast cancer and surrounding normal tissues is based on hydrophilic interaction liquid chromatography (HILIC)-electrospray ionization mass spectrometry (ESI-MS) quantitation of polar lipid classes of total lipid extracts followed by multivariate data analysis using unsupervised principal component analysis (PCA) and supervised orthogonal partial least square (OPLS). This analytical methodology is applied for the detailed lipidomic characterization of ten patients with the goal to find the statistically relevant differences between tumor and normal tissues. This strategy is selected for better visualization of differences, because the breast cancer tissue is compared with the surrounding healthy tissue of the same patient, therefore changes in the lipidome are caused predominantly by the tumor growth. A large increase of total concentrations for several lipid classes is observed, including phosphatidylinositols, phosphatidylethanolamines, phosphatidylcholines, and lysophosphatidylcholines. Concentrations of individual lipid species inside the abovementioned classes are also changed, and in some cases, these differences are statistically significant. PCA and OPLS analyses enable a clear differentiation of tumor and normal tissues based on changes of their lipidome. A notable decrease of relative abundances of ether and vinylether (plasmalogen) lipid species is detected for phosphatidylethanolamines, but no difference is apparent for phosphatidylcholines. en
utb.faculty Faculty of Humanities
dc.identifier.uri http://hdl.handle.net/10563/1004223
utb.identifier.obdid 43873545
utb.identifier.scopus 2-s2.0-84934451958
utb.identifier.wok 000348435600035
utb.identifier.pubmed 25352274
utb.identifier.coden ABCNB
utb.source j-wok
dc.date.accessioned 2015-05-06T06:58:34Z
dc.date.available 2015-05-06T06:58:34Z
dc.description.sponsorship ERC CZ project [LL1302]; Ministry of Education, Youth and Sports of the Czech Republic; [CZ.1.07/2.3.00/30.0021]
utb.contributor.internalauthor Gatěk, Jiří
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