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Characterization of keratin hydrolyzates prepared from sheep wool

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dc.title Characterization of keratin hydrolyzates prepared from sheep wool en
dc.contributor.author Mokrejš, Pavel
dc.contributor.author Krejčí, Ondřej
dc.contributor.author Sukop, Svatopluk
dc.contributor.author Svoboda (FT), Petr
dc.relation.ispartof Asian Journal of Chemistry
dc.identifier.issn 0970-7077 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2014
utb.relation.volume 26
utb.relation.issue 19
dc.citation.spage 6523
dc.citation.epage 6527
dc.type article
dc.language.iso en
dc.publisher Chemical Publishing Co.
dc.identifier.doi 10.14233/ajchem.2014.16480
dc.subject Hydrolysis en
dc.subject Hydrolyzate en
dc.subject Keratin en
dc.subject Molecular weight en
dc.subject Sheep wool en
dc.description.abstract Preparation of keratin hydrolyzates through alkaline-enzymatic hydrolysis was already described earlier. Employment of hydrolyzates in practice requires knowledge of their composition, thermal stability and molecular weight. The aim of this contribution is to determine molecular weight of keratin hydrolyzates (prepared from sheep wool). Molecular weight (Mw) of keratin hydrolyzates was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). Selection of hydrolysis conditions allows to prepare hydrolyzates with predominant share of low-molecular fractions (Mw< 10 kDa), intermediate-molecular fractions (Mw≈ 10-50 kDa) or high-molecular fractions (Mw> 70 kDa). Optimal conditions for preparing keratin hydrolyzates are as follows: in 1ststage of hydrolysis, acting on wool with 0.6% KOH at 90 °C for 48 h; in 2ndstage of hydrolysis, adding 1% (w/w) proteolytic enzyme Savinase 6 T and stirring at 40°C for 24 h. Under these conditions, breakdown efficiency is 63% and a hydrolyzate is prepared containing 11.5% N, 14.9% ash, 2.58% sulphur and a high portion of intermediate- and high-molecular fractions which is comparable in quality to technical gelatine. Keratin hydrolyzate can be employed, for example, to produce wrapping materials (films, coatings, encapsulates). en
utb.faculty University Institute
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1003869
utb.identifier.obdid 43872028
utb.identifier.scopus 2-s2.0-84907856073
utb.identifier.coden AJCHE
utb.source j-scopus
dc.date.accessioned 2014-11-19T09:23:50Z
dc.date.available 2014-11-19T09:23:50Z
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Mokrejš, Pavel
utb.contributor.internalauthor Krejčí, Ondřej
utb.contributor.internalauthor Sukop, Svatopluk
utb.contributor.internalauthor Svoboda (FT), Petr
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