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Development of an HPLC method for the determination of glycerol oxidation products

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dc.title Development of an HPLC method for the determination of glycerol oxidation products en
dc.contributor.author Beltrán-Prieto, Juan Carlos
dc.contributor.author Pecha, Jiří
dc.contributor.author Kašpárková, Věra
dc.contributor.author Kolomazník, Karel
dc.relation.ispartof Journal of Liquid Chromatography and Related Technologies
dc.identifier.issn 1082-6076 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2013
utb.relation.volume 36
utb.relation.issue 19
dc.citation.spage 2758
dc.citation.epage 2773
dc.type article
dc.language.iso en
dc.publisher Taylor and Francis Inc. en
dc.identifier.doi 10.1080/10826076.2012.725695
dc.relation.uri http://www.tandfonline.com/doi/abs/10.1080/10826076.2012.725695#.UkBkquR6nwM
dc.subject dihydroxyacetone en
dc.subject glyceraldehyde en
dc.subject glycerol oxidation en
dc.subject HPLC method en
dc.subject ion exchange en
dc.subject validation en
dc.description.abstract An HPLC method for the separation of glycerol oxidation products, namely glyceraldehyde, dihydroxyacetone, mesoxalic, tartronic, and glycolic and glyceric acids on an ion-exchange 8% cross-linked calcium sulfonated divinylbenzene-styrene resin column was developed and validated. The conditions reported include temperature (70°C), flow rate (0.5 mL/min) and concentration of the mobile phase (3 mM H2SO4) using isocratic elution with ultraviolet and refractometric detectors. The effect of the mobile phase flow rate and concentration as well as column temperature on the resolution of peaks is described. Excellent correlation coefficient in the calibration model was observed for all analytes over the concentration range of 0.5 to 10 mg/mL. The method was also validated in terms of intra-day precision, sensitivity, accuracy, and detection and quantification limits. The method conditions were applied to the identification of products derived from the chemical oxidation of glycerol. Supplemental materials are available for this article. Go to the publisher's online edition of the Journal of Liquid Chromatography & Related Technologies to view the supplemental file. © 2013 Taylor and Francis Group, LLC. en
utb.faculty Faculty of Applied Informatics
dc.identifier.uri http://hdl.handle.net/10563/1003461
utb.identifier.obdid 43869907
utb.identifier.scopus 2-s2.0-84882445077
utb.identifier.wok 000322865600009
utb.identifier.coden JLCTF
utb.source j-scopus
dc.date.accessioned 2013-09-20T08:46:16Z
dc.date.available 2013-09-20T08:46:16Z
utb.contributor.internalauthor Beltrán-Prieto, Juan Carlos
utb.contributor.internalauthor Pecha, Jiří
utb.contributor.internalauthor Kašpárková, Věra
utb.contributor.internalauthor Kolomazník, Karel
utb.fulltext.affiliation Juan Carlos Beltrán-Prieto*,1 Jiří Pecha,1 Věra Kašpárková, 2,3 and Karel Kolomazník1 1 Department of Automation and Control Engineering, Faculty of Applied Informatics, Tomas Bata University in Zlín, Zlín, Czech Republic 2 Center of Polymer Systems, University Institute, Tomas Bata University in Zlín, Zlín, Czech Republic 3 Department of Fat, Surfactant and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín, Zlín, Czech Republic *Address correspondence to Juan Carlos Beltrán-Prieto, Department of Automation and Control Engineering, Faculty of Applied Informatics, Tomas Bata University in Zlín, nam. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic. E-mail: prieto@fai.utb.cz
utb.fulltext.dates -
utb.fulltext.sponsorship The project was financially supported by the European Regional Development Fund (ERDF) under the Project CEBIA-Tech No. CZ.1.05=2.1.00= 03.0089, with support of Operational Program Research and Development for Innovations co-funded by the ERDF and national budget of Czech Republic, within the framework of project Center of Polymer Systems (reg. number: CZ.1.05=2.1.00=03.0111) and Tomas Bata University Internal Grant (IGA=FAI=2012=029). J. C. Beltrán-Prieto is also grateful for the doctoral scholarship provided by Tomas Bata University in Zlín and the National Council of Science and Technology (CONACYT) in Mexico.
utb.fulltext.faculty Faculty of Applied Informatics
utb.fulltext.faculty University Institute
utb.fulltext.faculty Faculty of Technology
utb.fulltext.ou Department of Automation and Control Engineering
utb.fulltext.ou Center of Polymer Systems
utb.fulltext.ou Department of Fat, Surfactant and Cosmetics Technology
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