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dc.title | High-performance liquid chromatographic method with amperometric detection employing boron-doped diamond electrode for the determination of sildenafil, vardenafil and their main metabolites in plasma | en |
dc.contributor.author | Bartošová, Zdenka | |
dc.contributor.author | Jirovský, David | |
dc.contributor.author | Horna, Aleš | |
dc.relation.ispartof | Journal of Chromatography A | |
dc.identifier.issn | 0021-9673 Scopus Sources, Sherpa/RoMEO, JCR | |
dc.date.issued | 2011 | |
utb.relation.volume | 1218 | |
utb.relation.issue | 44 | |
dc.citation.spage | 7996 | |
dc.citation.epage | 8001 | |
dc.type | article | |
dc.language.iso | en | |
dc.publisher | Elsevier Science B.V. | en |
dc.identifier.doi | 10.1016/j.chroma.2011.09.001 | |
dc.relation.uri | https://www.sciencedirect.com/science/article/pii/S0021967311013240 | |
dc.subject | amperometric detection | en |
dc.subject | boron-doped diamond electrode | en |
dc.subject | HPLC | en |
dc.subject | sildenafil | en |
dc.subject | vardenafil | en |
dc.description.abstract | A simple, fast and sensitive HPLC method with electrochemical detection employing boron-doped diamond electrode (BDD) for the determination of sildenafil (Viagra™), vardenafil (Levitra™) and their main metabolites, N-desmethyl sildenafil and N-desethyl vardenafil in human plasma is presented. The assay involved drug extraction by tert-butyl methyl ether and isocratic reversed-phase liquid chromatography with amperometric detection. Complete separation of all analytes was achieved within 12min. The mobile phase consisted of 20mM sodium dihydrogen phosphate with 40mM sodium perchlorate/acetonitrile (70:30, v/v), pH 3.5. The electrode working potential was +1520mV (vs. Pd/H2). Calibration curves were linear over the concentration range of 10-400ngmL-1. Phloretin was used as an internal standard. The limits of detection (LOD) and quantification (LOQ) for the studied analytes were within the range of 2-4ngmL-1 and 7.0-13.4ngmL-1, respectively. The developed method was applied to human plasma samples spiked with analytes at therapeutic concentrations. The study confirms the method's suitability for both pharmacokinetic studies and therapeutic monitoring. © 2011 Elsevier B.V. | en |
utb.faculty | University Institute | |
dc.identifier.uri | http://hdl.handle.net/10563/1002609 | |
utb.identifier.rivid | RIV/70883521:28610/11:43865692!RIV12-MSM-28610___ | |
utb.identifier.obdid | 43865708 | |
utb.identifier.scopus | 2-s2.0-80053941496 | |
utb.identifier.wok | 000296549000012 | |
utb.identifier.coden | JCRAE | |
utb.source | j-scopus | |
dc.date.accessioned | 2012-02-10T13:15:15Z | |
dc.date.available | 2012-02-10T13:15:15Z | |
utb.ou | Centre of Polymer Systems | |
utb.contributor.internalauthor | Horna, Aleš |