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Toxicological aspects of flavonoid interaction with biomacromolecules

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dc.title Toxicological aspects of flavonoid interaction with biomacromolecules en
dc.contributor.author Hodek, Petr
dc.contributor.author Hanuštiak, Pavel
dc.contributor.author Křížková, Jitka
dc.contributor.author Mikelová, Radka
dc.contributor.author Křížková, Soňa
dc.contributor.author Stiborová, Marie
dc.contributor.author Trnková, Libuše
dc.contributor.author Horna, Aleš
dc.contributor.author Beklová, Miroslava
dc.contributor.author Kizek, René
dc.relation.ispartof Neuroendocrinology Letters
dc.identifier.issn 0172-780X Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2006-12
utb.relation.volume 27
utb.relation.issue 2
dc.citation.spage 14
dc.citation.epage 17
dc.event.title 11th Interdisciplinary Slovak-Czech Toxicological Conference
dc.event.location Trenčianske Teplice
utb.event.state-en Slovakia
utb.event.state-cs Slovensko
dc.event.sdate 2006-06-05
dc.event.edate 2006-06-07
dc.type article
dc.type conferenceObject
dc.language.iso en
dc.publisher Maghira & Maas Publications en
dc.relation.uri http://data.radanal.cz/publikace/17_Hodek.pdf
dc.relation.uri http://web.archive.org/web/20071217154831/http://node.nel.edu/?node_id=3968
dc.subject rutin en
dc.subject quercetin en
dc.subject flavone en
dc.subject cytochrome P450 en
dc.subject western blotting en
dc.subject colon microsomes en
dc.subject cancer en
dc.subject DNA interaction en
dc.subject carbon paste electrode en
dc.subject square wave voltammetry en
dc.description.abstract OBJECTIVES: Flavonoids are widely accepted as health promoting phytochemicals, however, some flavonoids show ability of direct interaction with DNA and/or enhance carcinogen activation into DNA modifying agents. Thus, their potential harmful effect on the human body should be examined in detail. METHODS: Direct interaction of flavonoids (quercetin, rutin) with DNA was examined using square wave voltammetry on carbon paste electrode. Induction effect of selected flavonoids on content of cytochrome P450 1A1, carcinogens activating enzyme, in colon and liver microsomal samples of animals exposed to flavonoids was determined by Western blotting, using anti-cytochrome P450 lAl specific antibody. RESULTS: Of the natural flavonoids tested, induction of CYPIA1 was elicited by the typical citrus flavonoid naringenin in the colon, as well as by flavone in the liver. Moreover, synthetic (beta-naphthoflavone and naturally occurring chrysin, quercetin and diosmin induced CYP1A1 in both tissues. The oxidation signals of guanine and adenine in the DNA molecule were decreased in the presence of flavonoids. CONCLUSIONS: Although flavonoids are often considered to be safe because of their "plant origin"; ingestion of flavonoids should be taken with caution. Enhanced expression of CYPIA1 in colon tissue might be responsible for increasing incidence of colorectal carcinoma in humans. Electrochemistry can be used to study the interactions of flavonoids and DNA. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1001947
utb.identifier.obdid 43865433
utb.identifier.scopus 2-s2.0-34047217681
utb.identifier.wok 000245442600004
utb.identifier.coden NLETD
utb.source d-wok
dc.date.accessioned 2011-08-09T07:34:17Z
dc.date.available 2011-08-09T07:34:17Z
utb.contributor.internalauthor Horna, Aleš
utb.fulltext.affiliation Petr Hodek1, Pavel Hanuštiak2,4, Jitka Křížková1, Radka Mikelová3, Soňa Křížková2, Marie Stiborová1, Libuše Trnková3, Aleš Horna5, Miroslava Beklová4 & René Kizek2 1. Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic 2. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic 3. Department of Theoretical and Physical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic 4. Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 5. Department of Food Engineering and Chemistry, Faculty of Technology, Tomas Bata University, Zlin, Czech Republic Correspondence to: Assoc. Prof. RNDr. Petr Hodek, PhD. Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 2030, 128 40 Prague 2, Czech Republic TEL.: +420-2-21951285, FAX: +420-2-21951283 EMAIL: hodek@natur.cuni.cz
utb.fulltext.dates Submitted: September 18, 2006 Accepted: November 2, 2006
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utb.fulltext.sponsorship The financial support from grants 303/06/0928, 525/04/P132 and A100040602 from the Grant Agency of the Czech Republic, and 1M4635608802 from the Czech Ministry of Education is highly acknowledged.
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