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Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection

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dc.title Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection en
dc.contributor.author Stejskal, Karel
dc.contributor.author Křížková, Soňa
dc.contributor.author Adam, Vojtěch
dc.contributor.author Sures, Bernd
dc.contributor.author Trnková, Libuše
dc.contributor.author Zehnálek, Josef
dc.contributor.author Hubálek, Jaromír
dc.contributor.author Beklová, Miroslava
dc.contributor.author Hanuštiak, Pavel
dc.contributor.author Svobodová, Zdenka
dc.contributor.author Horna, Aleš
dc.contributor.author Kizek, René
dc.relation.ispartof IEEE Sensors Journal
dc.identifier.issn 1530-437X Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2008-09/2008-10
utb.relation.volume 8
utb.relation.issue 9-10
dc.citation.spage 1578
dc.citation.epage 1585
dc.event.title 5th IEEE Sensors Conference
dc.event.location Daegu
utb.event.state-en Democratic People's Republic of Korea
utb.event.state-cs Severní Korea
dc.event.sdate 2006-10-22
dc.event.edate 2006-10-25
dc.type article
dc.type conferenceObject
dc.language.iso en
dc.publisher The Institute of Electrical and Electronics Engineers (IEEE) en
dc.identifier.doi 10.1109/JSEN.2008.928500
dc.relation.uri http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=4609975
dc.subject Amperometric detection en
dc.subject biomarker en
dc.subject electrochemical sensors en
dc.subject fish en
dc.subject flow injection analysis en
dc.subject heavy metal en
dc.subject metallothionein en
dc.subject poecilia reticulata en
dc.subject silver en
dc.description.abstract In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, "current R": 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled with differential pulse voltammetry Brdicka reaction, which was used as the reference technique. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1001850
utb.identifier.obdid 43857023
utb.identifier.obdid 43857370
utb.identifier.scopus 2-s2.0-85008013885
utb.identifier.wok 000260384900014
utb.source d-wok
dc.date.accessioned 2011-08-09T07:34:05Z
dc.date.available 2011-08-09T07:34:05Z
utb.contributor.internalauthor Horna, Aleš
utb.fulltext.affiliation Karel Stejskal, Sona Krizkova, Vojtech Adam, Bernd Sures, Libuse Trnkova, Josef Zehnalek, Jaromir Hubalek, Miroslava Beklova, Pavel Hanustiak, Zdenka Svobodova, Ales Horna, and Rene Kizek K. Stejskal is with the Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic and also with the Department of Biochemistry, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech Republic. S. Krizkova, J. Zehnalek, and R. Kizek are with the Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic (e-mail: kizek@sci.muni.cz). V. Adam is with the Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic and also with the Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, CZ-613 00 Brno, Czech Republic. B. Sures is with the Universität Duisburg-Essen, Applied Zoology and Hydrobiology, D-45117 Essen, Germany. L. Trnkova is with the Department of Chemistry, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech Republic. J. Hubalek is with the Department of Microelectronics, Faculty of Electrical Engineering and Communication, Technical University of Brno, CZ-602 00 Brno, Czech Republic. M. Beklova and P. Hanustiak are with the Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, CZ-612 42 Brno, Czech Republic. Z. Svobodova is with the Institute of Parasitology, University of Veterinary and Pharmaceutical Sciences, CZ-612 42 Brno, Czech Republic. A. Horna is with the Faculty of Technology, Tomas Bata University, CZ-762 72 Zlin, Czech Republic.
utb.fulltext.dates Manuscript received March 31, 2007; revised June 30, 2007 and September 28, 2007; accepted January 6, 2008.
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utb.fulltext.sponsorship This work was supported under Grants GACR 526/07/0808, MSMT 6215712402, MSMT 2636/F4b, and MSMT INCHEMBIOL 0021622412.
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