The effect of plasma pretreatment and cross-linking degree on the physical and antimicrobial properties of nisin-coated PVA films

Kolářová Rašková, Zuzana; Sťahel, Pavel; Sedlaříková, Jana; Musilová, Lenka; Stupavská, Monika; Lehocký, Marián

dc.title	The effect of plasma pretreatment and cross-linking degree on the physical and antimicrobial properties of nisin-coated PVA films	en
dc.contributor.author	Kolářová Rašková, Zuzana
dc.contributor.author	Sťahel, Pavel
dc.contributor.author	Sedlaříková, Jana
dc.contributor.author	Musilová, Lenka
dc.contributor.author	Stupavská, Monika
dc.contributor.author	Lehocký, Marián
dc.relation.ispartof	Materials
dc.identifier.issn	1996-1944 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2018
utb.relation.volume	11
utb.relation.issue	8
dc.type	article
dc.language.iso	en
dc.publisher	MDPI AG
dc.identifier.doi	10.3390/ma11081451
dc.relation.uri	http://www.mdpi.com/1996-1944/11/8/1451
dc.subject	antimicrobial film	en
dc.subject	nisin	en
dc.subject	physical properties	en
dc.subject	plasma treatment polyvinyl alcohol	en
dc.subject	surface characterization	en
dc.description.abstract	Stable antimicrobial nisin layers were prepared on the carrying medium-polyvinyl alcohol (PVA) films, crosslinked by glutaric acid. Surface plasma dielectric coplanar surface barrier discharge (DCSBD) modification of polyvinyl alcohol was used to improve the hydrophilic properties and to provide better adhesion of biologically active peptide-nisin to the polymer. The surface modification of films was studied in correlation to their cross-linking degree. Nisin was attached directly from the salt solution of the commercial product. In order to achieve a stable layer, the initial nisin concentration and the following release were investigated using chromatographic methods. The uniformity and stability of the layers was evaluated by means of zeta potential measurements, and for the surface changes of hydrophilic character, the water contact angle measurements were provided. The nisin long-term stability on the PVA films was confirmed by tricine polyacrylamide gel electrophoresis (SDS-PAGE) and by antimicrobial assay. It was found that PVA can serve as a suitable carrying medium for nisin with tunable properties by plasma treatment and crosslinking degree. © 2018 by the authors.	en
utb.faculty	University Institute
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1008161
utb.identifier.obdid	43879688
utb.identifier.scopus	2-s2.0-85051776514
utb.identifier.wok	000444112800188
utb.identifier.pubmed	30115861
utb.source	j-scopus
dc.date.accessioned	2018-08-30T13:31:22Z
dc.date.available	2018-08-30T13:31:22Z
dc.description.sponsorship	Czech Science Foundation (Grant Agency of the Czech Republic) [17-10813S]
dc.rights	Attribution 4.0 International
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.rights.access	openAccess
utb.ou	Centre of Polymer Systems
utb.contributor.internalauthor	Kolářová Rašková, Zuzana
utb.contributor.internalauthor	Sedlaříková, Jana
utb.contributor.internalauthor	Musilová, Lenka
utb.contributor.internalauthor	Lehocký, Marián
utb.fulltext.affiliation	Zuzana Kolarova Raskova 1,* https://orcid.org/0000-0001-5323-8053 , Pavel Stahel 2, Jana Sedlarikova 1,3, Lenka Musilova 1, Monika Stupavska 2 and Marian Lehocky 1,3 1 Centre of polymer systems, Tomas Bata University, Trida Tomase Bati 5678, 76001 Zlin, Czech Republic; sedlarikova@utb.cz (J.S.); musilova@utb.cz (L.M.); lehocky@utb.cz (M.L.) 2 Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlarska 267/2, 63711 Brno, Czech Republic; pstahel@physics.muni.cz (P.S.); 119414@mail.muni.cz (M.S.) 3 Department of Fat, Surfactants and Cosmetics Technology, Faculty of Technology, Tomas Bata University, Vavrečkova 275, 76001 Zlin, Czech Republic * Correspondence: zraskova@cps.utb.cz; Tel.: +420-724-333-988
utb.fulltext.dates	Received: 13 July 2018 Accepted: 13 August 2018 Published: 16 August 2018
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utb.fulltext.sponsorship	This work was supported by the grant 17-10813S of the Czech Science Foundation (Grant Agency of the Czech Republic).
utb.wos.affiliation	[Raskova, Zuzana Kolarova; Sedlarikova, Jana; Musilova, Lenka; Lehocky, Marian] Tomas Bata Univ, Ctr Polymer Syst, Trida Tomase Bati 5678, Zlin 76001, Czech Republic; [Stahel, Pavel; Stupavska, Monika] Masaryk Univ, Fac Sci, Dept Phys Elect, Kotlarska 267-2, Brno 63711, Czech Republic; [Sedlarikova, Jana; Lehocky, Marian] Tomas Bata Univ, Fac Technol, Dept Fat Surfactants & Cosmet Technol, Vavreckova 275, Zlin 76001, Czech Republic
utb.scopus.affiliation	Centre of polymer systems, Tomas Bata University, Trida Tomase Bati 5678, Zlin, Czech Republic; Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlarska 267/2, Brno, Czech Republic; Department of Fat, Surfactants and Cosmetics Technology, Faculty of Technology, Tomas Bata University, Vavrečkova 275, Zlin, Czech Republic
utb.fulltext.projects	17-10813S