Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars

Sochor, Jiří; Škutková, Helena; Babula, Petr; Zítka, Ondřej; Cernei, Natalia; Rop, Otakar; Krška, Boris; Adam, Vojtěch; Provazník, Ivo; Kizek, René

dc.title	Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars	en
dc.contributor.author	Sochor, Jiří
dc.contributor.author	Škutková, Helena
dc.contributor.author	Babula, Petr
dc.contributor.author	Zítka, Ondřej
dc.contributor.author	Cernei, Natalia
dc.contributor.author	Rop, Otakar
dc.contributor.author	Krška, Boris
dc.contributor.author	Adam, Vojtěch
dc.contributor.author	Provazník, Ivo
dc.contributor.author	Kizek, René
dc.relation.ispartof	Molecules
dc.identifier.issn	1420-3049 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2011
utb.relation.volume	16
utb.relation.issue	9
dc.citation.spage	7428
dc.citation.epage	7457
dc.type	article
dc.language.iso	en
dc.publisher	MDPI AG	en
dc.identifier.doi	10.3390/molecules16097428
dc.relation.uri	http://www.mdpi.com/1420-3049/16/9/7428/
dc.subject	amino acids	en
dc.subject	antioxidant activity	en
dc.subject	apricot	en
dc.subject	polyphenolics	en
dc.subject	statistical analysis	en
dc.description.abstract	Functional foods are of interest because of their significant effects on human health, which can be connected with the presence of some biologically important compounds. In this study, we carried out complex analysis of 239 apricot cultivars (Prunus armeniaca L.) cultivated in Lednice (climatic area T4), South Moravia, Czech Republic. Almost all previously published studies have focused only on analysis of certain parameters. However, we focused on detection both primary and secondary metabolites in a selection of apricot cultivars with respect to their biological activity. The contents of thirteen biogenic alpha-L-amino acids (arginine, asparagine, isoleucine, lysine, serine, threonine, valine, leucine, phenylalanine, tryptophan, tyrosine, proline and alanine) were determined using ion exchange chromatography with UV-Vis spectrometry detection. Profile of polyphenols, measured as content of ten polyphenols with significant antioxidant properties (gallic acid, procatechinic acid, p-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferrulic acid and quercetrin), was determined by high performance liquid chromatography with spectrometric/electrochemical detection. Moreover, content of total phenolics was determined spectrophotometrically using the Folin-Ciocalteu method. Antioxidant activity was determined using five independent spectrophotometric methods: DPPH assay, DMPD method, ABTS method, FRAP and Free Radicals methods. Considering the complexity of the obtained data, they were processed and correlated using bioinformatics techniques (cluster analysis, principal component analysis). The studied apricot cultivars were clustered according to their common biochemical properties, which has not been done before. The observed similarities and differences were discussed. Â© 2011 by The Authors.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1002656
utb.identifier.rivid	RIV/70883521:28110/11:43865587!RIV12-MSM-28110___
utb.identifier.obdid	43865599
utb.identifier.scopus	2-s2.0-80053290789
utb.identifier.wok	000295211000020
utb.identifier.coden	MOLEF
utb.source	j-scopus
dc.date.accessioned	2012-02-10T13:15:25Z
dc.date.available	2012-02-10T13:15:25Z
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.access	openAccess
utb.contributor.internalauthor	Rop, Otakar
utb.fulltext.affiliation	Jiri Sochor 1, Helena Skutkova 2, Petr Babula 1, Ondrej Zitka 1, Natalia Cernei 1, Otakar Rop 3, Boris Krska 4, Vojtech Adam 1,5, Ivo Provazník 2 and Rene Kizek 1,5,* 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Kolejni 4, CZ-612 00 Brno, Czech Republic 3 Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlin, Namesti T. G. Masaryka 275, CZ-762 72 Zlin, Czech Republic 4 Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Valticka 337, CZ-691 44 Lednice, Czech Republic 5 Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic * Author to whom correspondence should be addressed; E-Mail: kizek@sci.muni.cz;Tel.: +420-5-4513-3350; Fax: +420-5-4521-2044.
utb.fulltext.dates	Received: 10 August 2011 Accepted: 17 August 2011 Published: 1 September 2011
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