Determination of isoflavones using liquid chromatography with electrochemical detection

Abstract

Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min(-1); column and detector temperature: 26 degrees C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL(-1). Their simultaneous determination takes 15 min.