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Expression of oxysterol pathway genes in oestrogen-positive breast carcinomas

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dc.title Expression of oxysterol pathway genes in oestrogen-positive breast carcinomas en
dc.contributor.author Kloudová, Alžběta
dc.contributor.author Brynychová, Veronika
dc.contributor.author Václavíková, Radka
dc.contributor.author Vrána, David
dc.contributor.author Gatěk, Jiří
dc.contributor.author Mrhalová, Marcela
dc.contributor.author Kodet, Roman
dc.contributor.author Souček, Pavel
dc.relation.ispartof Clinical Endocrinology
dc.identifier.issn 0300-0664 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2017
utb.relation.volume 86
utb.relation.issue 6
dc.citation.spage 852
dc.citation.epage 861
dc.type article
dc.language.iso en
dc.publisher Blackwell Publishing Ltd.
dc.identifier.doi 10.1111/cen.13337
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1111/cen.13337/full
dc.subject breast carcinoma en
dc.subject expression en
dc.subject oestrogen receptor en
dc.subject oxysterols en
dc.subject prognosis en
dc.description.abstract Objective: This study investigated whether gene expression levels of key modulators of the oxysterol signalling pathway modify the prognosis of patients with oestrogen receptor-positive (ER+) breast carcinomas via interaction with endocrine therapy. Context: The prognosis of patients with ER+ breast carcinoma depends on several factors. Previous studies have suggested that some oxygenated forms of cholesterol (oxysterols) bind to oestrogen receptor and anti-oestrogen binding site which may deregulate cholesterol homoeostasis and influence effect of therapy. Design: The expression levels of 70 oxysterol pathway genes were evaluated in a test set of breast carcinomas differing in ER expression. The genes differentially expressed in ER+ tumours were assessed in a comprehensive set of ER+ tumours to evaluate their clinical significance. Patients: A total of 193 primary patients with breast carcinoma were included. Measurements: The transcript levels were determined by quantitative real-time polymerase chain reaction. Results: The expression levels of 23 genes were found to be specifically dysregulated in ER+ tumours compared to ER− tumours of the test set. The expression levels of ABCG2, CYP7B1, CYP24A1, CYP39A1 and CH25H genes were found to be strongly associated with disease stage; however, none of the gene expression levels were associated with disease-free survival in patients treated with endocrine therapy. Conclusions: The expression of a number of oxysterol pathway genes is significantly modulated by ER expression and associated with the clinical stage of patients. However, the expression of oxysterol pathway genes was not found to modify the prognosis of ER+ patients with breast carcinoma treated with endocrine therapy. © 2017 John Wiley & Sons Ltd en
utb.faculty Faculty of Humanities
dc.identifier.uri http://hdl.handle.net/10563/1007355
utb.identifier.obdid 43876610
utb.identifier.scopus 2-s2.0-85018900712
utb.identifier.wok 000403714100013
utb.identifier.pubmed 28342201
utb.identifier.coden CLENA
utb.source j-scopus
dc.date.accessioned 2017-09-08T12:14:44Z
dc.date.available 2017-09-08T12:14:44Z
dc.description.sponsorship 15-25618A, MZCR, Ministerstvo Zdravotnictví Ceské Republiky
dc.description.sponsorship Grantova Agentura Ceske Republiky [13-25222J]; Ministry of Health of the Czech Republic [15-25618A]; National Sustainability Program I [LO1503]
utb.contributor.internalauthor Gatěk, Jiří
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