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High-performance liquid chromatographic method with amperometric detection employing boron-doped diamond electrode for the determination of sildenafil, vardenafil and their main metabolites in plasma

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dc.title High-performance liquid chromatographic method with amperometric detection employing boron-doped diamond electrode for the determination of sildenafil, vardenafil and their main metabolites in plasma en
dc.contributor.author Bartošová, Zdenka
dc.contributor.author Jirovský, David
dc.contributor.author Horna, Aleš
dc.relation.ispartof Journal of Chromatography A
dc.identifier.issn 0021-9673 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued 2011
utb.relation.volume 1218
utb.relation.issue 44
dc.citation.spage 7996
dc.citation.epage 8001
dc.type article
dc.language.iso en
dc.publisher Elsevier Science B.V. en
dc.identifier.doi 10.1016/j.chroma.2011.09.001
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S0021967311013240
dc.subject Amperometric detection en
dc.subject Boron-doped diamond electrode en
dc.subject HPLC en
dc.subject Sildenafil en
dc.subject Vardenafil en
dc.description.abstract A simple, fast and sensitive HPLC method with electrochemical detection employing boron-doped diamond electrode (BDD) for the determination of sildenafil (Viagra™), vardenafil (Levitra™) and their main metabolites, N-desmethyl sildenafil and N-desethyl vardenafil in human plasma is presented. The assay involved drug extraction by tert-butyl methyl ether and isocratic reversed-phase liquid chromatography with amperometric detection. Complete separation of all analytes was achieved within 12min. The mobile phase consisted of 20mM sodium dihydrogen phosphate with 40mM sodium perchlorate/acetonitrile (70:30, v/v), pH 3.5. The electrode working potential was +1520mV (vs. Pd/H2). Calibration curves were linear over the concentration range of 10-400ngmL-1. Phloretin was used as an internal standard. The limits of detection (LOD) and quantification (LOQ) for the studied analytes were within the range of 2-4ngmL-1 and 7.0-13.4ngmL-1, respectively. The developed method was applied to human plasma samples spiked with analytes at therapeutic concentrations. The study confirms the method's suitability for both pharmacokinetic studies and therapeutic monitoring. © 2011 Elsevier B.V. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1002609
utb.identifier.rivid RIV/70883521:28610/11:43865692!RIV12-MSM-28610___
utb.identifier.obdid 43865708
utb.identifier.scopus 2-s2.0-80053941496
utb.identifier.wok 000296549000012
utb.identifier.coden JCRAE
utb.source j-scopus
dc.date.accessioned 2012-02-10T13:15:15Z
dc.date.available 2012-02-10T13:15:15Z
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Horna, Aleš
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