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Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on glassy carbon electrode

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dc.title Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on glassy carbon electrode en
dc.contributor.author Zítka, Ondřej
dc.contributor.author Horna, Aleš
dc.contributor.author Stejskal, Karel
dc.contributor.author Zehnálek, Josef
dc.contributor.author Adam, Vojtěch
dc.contributor.author Havel, Ladislav
dc.contributor.author Zeman, Ladislav
dc.contributor.author Kizek, René
dc.relation.ispartof Acta Chimica Slovenica
dc.identifier.issn 1318-0207 OCLC, Ulrich, Sherpa/RoMEO, JCR
dc.date.issued 2007
utb.relation.volume 54
utb.relation.issue 1
dc.citation.spage 68
dc.citation.epage 73
dc.event.title 12th International Symposium on Sparation Sciences
dc.event.location Lipica
utb.event.state-en Slovakia
utb.event.state-cs Slovensko
dc.event.sdate 2006-09-27
dc.event.edate 2006-09-29
dc.type article
dc.type conferenceObject
dc.language.iso en
dc.publisher Slovensko kemijsko društvo sk
dc.relation.uri http://acta.chem-soc.si/54/54-1-68.pdf
dc.subject lactoferrin en
dc.subject milk protein en
dc.subject linear sweep voltammetry en
dc.subject differential pulse voltammetry en
dc.subject square wave voltammetry en
dc.subject carbon paste electrode en
dc.subject glassy carbon electrode en
dc.description.abstract Lactoferrin is considered to be a multifunctional protein. It appears to play several biologically important roles, where its structure is very crucial. The aim of this work was to investigate the basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect its structural changes under various denaturing conditions. The electroactivity of lactoferrin was studied by three various electrochemical methods ( linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection (FIA-ED) for determination of lactoferrin. We found out that the most suitable FIA-ED conditions were as follows: working electrode potential of 900 mV, Britton-Robinson buffer (pH 4.5) as the mobile phase, its flow rate 1 ml min(-1). Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to changes in its structure. As we have shown here, electrochemical analysis enables us to distinguish changes of protein structure easily and rapidly. en
utb.faculty Faculty of Technology
dc.identifier.uri http://hdl.handle.net/10563/1001919
utb.identifier.obdid 43865796
utb.identifier.scopus 2-s2.0-34247497678
utb.identifier.wok 000245178800010
utb.source d-wok
dc.date.accessioned 2011-08-09T07:34:14Z
dc.date.available 2011-08-09T07:34:14Z
utb.contributor.internalauthor Horna, Aleš
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