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CoulArray Detector as a Tool for Estimation of Acute Toxicity of Silver(I) Ions

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dc.title CoulArray Detector as a Tool for Estimation of Acute Toxicity of Silver(I) Ions en
dc.contributor.author Húska, Dalibor
dc.contributor.author Adam, Vojtěch
dc.contributor.author Horna, Aleš
dc.contributor.author Svobodová, Zdenka
dc.contributor.author Zítka, Ondřej
dc.contributor.author Beklová, Miroslava
dc.contributor.author Kizek, René
dc.relation.ispartof International Journal of Electrochemical Science
dc.identifier.issn 1452-3981 OCLC, Ulrich, Sherpa/RoMEO, JCR
dc.date.issued 2010
utb.relation.volume 5
utb.relation.issue 8
dc.citation.spage 1082
dc.citation.epage 1089
dc.type article
dc.language.iso en
dc.publisher Electrochemical Science Group/Electrochemical Society, Inc. en
dc.relation.uri http://www.electrochemsci.org/papers/vol5/5081082.pdf
dc.subject heavy metal en
dc.subject silver en
dc.subject bioaccumulation en
dc.subject aquatic organisms en
dc.subject electrochemical detector en
dc.subject heavy-metal ions en
dc.subject liquid-chromatography en
dc.subject electrochemical detection en
dc.subject environmental-pollution en
dc.subject metallothionein level en
dc.subject oxidized glutathione en
dc.subject biochemical markers en
dc.subject carbon nanotubes en
dc.subject array detection en
dc.subject plant thiols en
dc.description.abstract Biochemical markers suitable for monitoring of environmental pollution as well as for protecting of human health are searching. In this study, the effect of silver(I) ions on guppy fishes (Poecilia reticulata) was investigated. For this purpose, we employed hyphenated technique of high performance liquid chromatography coupled with multichannel CoulArray electrochemical detector. Content of cysteine in fishes treated with 0.5 and/or 1 mu g/l of silver(I) ions was 290 and/or 240 ng/g at the end of seven day long treatment. Reduced and oxidized glutathione were also determined. The enhance in GSSG content accompanied by decrease in GSH content confirms our hypothesis on increasing risk of oxidative stress in fishes due to treatment with silver(I) ions. In addition, we attempted to gain more information about health state of animals exposed to heavy metals from chromatograms measured by CoulArray detector. We counted areas of all signals detected up to 30 minutes in chromatograms of tissues from fishes treated with all concentrations of silver(I) ions. The content of thiols and thiols related compounds as a sum of signals areas enhanced with increasing concentration of silver(I) ions and time of exposition. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1001715
utb.identifier.rivid RIV/70883521:28610/10:63509222!RIV11-MSM-28610___
utb.identifier.obdid 43864575
utb.identifier.scopus 2-s2.0-77956855880
utb.identifier.wok 000281951900003
utb.source j-riv
dc.rights Attribution 4.0 International
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.rights.access openAccess
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Horna, Aleš
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International