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Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

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dc.title Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions en
dc.contributor.author Diopan, Václav
dc.contributor.author Shestivska, Violetta
dc.contributor.author Galiová, Michaela
dc.contributor.author Adam, Vojtěch
dc.contributor.author Kaiser, Jozef
dc.contributor.author Horna, Aleš
dc.contributor.author Novotný, Karel
dc.contributor.author Liska, Miroslav
dc.contributor.author Havel, Ladislav
dc.contributor.author Zehnálek, Josef
dc.contributor.author Zítka, Ondřej
dc.contributor.author Kizek, René
dc.relation.ispartof Plasma Processes and Polymers
dc.identifier.issn 1612-8850 OCLC, Ulrich, Sherpa/RoMEO, JCR
dc.date.issued 2010
utb.relation.volume 22
utb.relation.issue 11
dc.citation.spage 1248
dc.citation.epage 1259
dc.type article
dc.language.iso en
dc.publisher Wiley-VCH Verlag GmbH & Co. en
dc.identifier.doi 10.1002/elan.200900374
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1002/elan.200900374/pdf
dc.subject transfer stripping technique en
dc.subject heavy-metal detoxification en
dc.subject electrochemical detection en
dc.subject oxidized glutathione en
dc.subject fluorescence detection en
dc.subject capillary-electrophoresis en
dc.subject mass-spectrometry en
dc.subject brdicka reaction en
dc.subject toxic metals en
dc.subject waste-water en
dc.subject thiols en
dc.subject liquid chromatography en
dc.subject coulometric detection en
dc.subject amperometric detection en
dc.subject heavy metal en
dc.subject lased induced breakdown spectroscopy en
dc.description.abstract The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead (II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperomterich detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower n comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy. en
utb.faculty University Institute
dc.identifier.uri http://hdl.handle.net/10563/1001711
utb.identifier.rivid RIV/70883521:28610/10:63509218!RIV11-MSM-28610___
utb.identifier.obdid 43864559
utb.identifier.scopus 2-s2.0-77953302313
utb.identifier.wok 000278850200013
utb.source j-riv
utb.ou Centre of Polymer Systems
utb.contributor.internalauthor Horna, Aleš
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